Dmrt7在绵羊不同发育期睾丸组织中的表达与定位

doi:10.11843/j.issn.0366-6964.2016.12.008

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (12): 2390-2397.doi: 10.11843/j.issn.0366-6964.2016.12.008

Dmrt7在绵羊不同发育期睾丸组织中的表达与定位

李讨讨^1,2，郭亚敏¹，马友记^1,2*，赵兴绪³，卢曾奎¹，雒瑞瑞¹，高建锋¹

(1.甘肃农业大学动物科学技术学院,兰州 730070； 2.甘肃肉羊繁育生物技术工程实验室,民勤 733300；3.甘肃农业大学动物医学院,兰州 730070)

收稿日期:2016-07-28 出版日期:2016-12-23 发布日期:2016-12-23
通讯作者: 马友记，教授，主要从事绵山羊繁育与疫病防治研究，E-mail：yjma@gsau.edu.cn
作者简介:李讨讨(1992-)，甘肃庆阳人，硕士，主要从事羊生产系统与工程的研究，E-mail：1374658988@qq.com
基金资助:
甘肃省科技重大专项计划（1602NKDH020）；甘肃省高校基本科研业务费（2014）；甘肃省农业生物技术研究与应用开发项目(GNSW-2012-19)；国家肉羊产业技术体系项目（CARS-39）

Expression and Cellular Localization of Dmrt7 in Testis of Sheep at Different Developmental Stages

LI Tao-tao^{1, 2}，GUO Ya-min¹，MA You-ji^{1, 2*}，ZHAO Xing-xu³，LU Zeng-kui¹，LUO Rui-rui¹， GAO Jian-feng¹

(1.College of Animal Science and Technology，Gansu Agricultural University，Lanzhou 730070， China；2.Sheep Breeding Biotechnology Engineering Laboratory of Gansu Province，Minqin 733300，China；3.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China)

Received:2016-07-28 Online:2016-12-23 Published:2016-12-23

摘要/Abstract

摘要：

探讨Double sex和mab-3相关转录因子7（Double sex and mab-3 related transcription factor7，Dmrt7）在不同发育期绵羊睾丸组织中的表达规律和定位情况，本试验选取0、2、6、12和24月龄健康绵羊15只，每组3只，采集睾丸组织，运用实时荧光定量PCR、Western blot和免疫组织化学染色方法检测不同月龄睾丸组织中Dmrt7的表达与定位情况。结果显示，在不同月龄绵羊睾丸组织中均检测到Dmrt7 mRNA和蛋白，并且表达趋势较为一致，但不同月龄睾丸中表达量存在差异；在0、2和6月龄绵羊睾丸中，Dmrt7 mRNA和蛋白表达量很低，而在12和24月龄表达量较高，并且极显著高于0、2和6月龄（P<0.01）；0、2和6月龄绵羊睾丸组织中免疫阳性反应定位于少量的精原细胞，而在12和24月龄睾丸组织中免疫阳性反应主要定位于次级精母细胞和部分初级精母细胞及精子细胞。研究结果表明,Dmrt7可能对各级生精细胞的分裂过程尤其是初级精母细胞通过两次减数分裂形成精子细胞的过程起着重要的调控作用。

Abstract:

In order to understand the expression and cellular localization of double sex and mab-3 related transcription factor 7 (Dmrt7) in Small-Tail Han sheep testis tissues, fifteen sheep testis tissues were collected at 0, 2, 6, 12 and 24-month-old stages (3 replicates each), and the expression and cellular localization of Dmrt7 on testis tissues were detected by qRT-PCR, Western blot and immunohistochemistry. The results showed that Dmrt7 mRNA and protein were detected in sheep testes at different months of age, and the change pattern was similar. But, there were differences concerning the expression level of Dmrt7 for the different month groups. Dmrt7 mRNA and protein were lowly expressed in 0, 2 and 6 -month-old sheep testes, but it were highly expressed in 12, 24-month-old sheep testes. Meanwhile, the expression of Dmrt7 mRNA and protein in 12 and 24-month-old sheep testis tissues were extremely significantly higher than those in 0, 2 and 6-month-old sheep testes tissues（P<0.01）. The immunohistochemically staining revealed that immunoreactivity of Dmrt7 protein was localized to minority of spermatogonia in 0, 2 and 6-month-old sheep testis tissues, but it was mainly localized to secondary spermatocytes and partial primary spermatocytes and spermatids in 12 and 24-month-old sheep testis tissues. The results demonstrated that Dmrt7 played an essential regulation role to the division processes of various spermatogenic cells in testicular seminiferous tubule, especially meiotic division from primary spermatocytes to spermatids.

中图分类号:

李讨讨，郭亚敏，马友记，赵兴绪，卢曾奎，雒瑞瑞，高建锋. Dmrt7在绵羊不同发育期睾丸组织中的表达与定位[J]. 畜牧兽医学报, 2016, 47(12): 2390-2397.

LI Tao-tao，GUO Ya-min，MA You-ji，ZHAO Xing-xu，LU Zeng-kui，LUO Rui-rui， GAO Jian-feng. Expression and Cellular Localization of Dmrt7 in Testis of Sheep at Different Developmental Stages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2390-2397.

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Dmrt7在绵羊不同发育期睾丸组织中的表达与定位

Expression and Cellular Localization of Dmrt7 in Testis of Sheep at Different Developmental Stages

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