构建具有毛囊结构的组织工程皮肤及移植研究

doi:10.11843/j.issn.0366-6964.2019.10.016

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (10): 2097-2104.doi: 10.11843/j.issn.0366-6964.2019.10.016

构建具有毛囊结构的组织工程皮肤及移植研究

史明艳^1,2, 高雪^1*, 杨学义², 窦忠英³, 许尚忠¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 洛阳师范学院生命科学学院, 洛阳 471934;
3. 西北农林科技大学国家干细胞工程技术研究中心陕西分中心, 杨凌 712100

收稿日期:2019-04-24 出版日期:2019-10-23 发布日期:2019-10-23
通讯作者: 高雪,主要从事牛遗传育种与功能基因组学研究,E-mail:gaoxue76@126.com
作者简介:史明艳(1976-),女,河南林州人,博士,副教授,主要从事哺乳动物干细胞方面的研究,E-mail:smy2003@sina.com
基金资助:
NSFC-河南人才培养联合基金项目（U1504323）；国家自然科学基金面上项目（31572376）；中国农业科学院基本科研增量项目（Y2016PT17）；中国农业科学院创新工程项目（ASTIP-IAS03）

Construction and Transplantation of Tissue Engineered Skin with Hair Follicles

SHI Mingyan^1,2, GAO Xue^1*, YANG Xueyi², DOU Zhongying³, XU Shangzhong¹

1. Laboratory of Molecular Biology and Bovine Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Life Science, Luoyang Normal University, Luoyang 471934, China;
3. Shaanxi Branch of National Stem Cell Engineering Center, Northwest A & F University, Yangling 712100, China

Received:2019-04-24 Online:2019-10-23 Published:2019-10-23

摘要/Abstract

摘要： 旨在构建具有毛囊、汗腺等皮肤附属器官的组织工程皮肤，为人类毛发移植和受损皮肤修复的临床应用提供新的思路。试验以山羊毛囊干细胞和真皮细胞为种子细胞，以羊膜、Ⅰ型胶原为支架材料，进行组织工程皮肤的体外构建，并将组织工程皮肤移植到山羊体内（试验组，同时以羊膜代替组织工程皮肤进行移植作为对照组），观察和检测其对受损皮肤的修复效果。试验结果表明，人工构建的组织工程皮肤经体外培养25 d后，发现有毛囊结构形成；人工皮肤经扫描电镜观察，显示表皮细胞出现角质化；移植30、60、90 d后的瘢痕面积显示，试验组创面恢复平整，瘢痕面积极显著小于对照组（P<0.01）；移植270 d后，活体组织切片检测发现，试验组真、表皮结构已发生重建，可见分化明显的基底层、毛囊结构，而对照组为致密的结缔组织。综上表明，本研究首次以山羊毛囊干细胞和毛囊真皮细胞为种子细胞，成功构建了具有毛囊器官的组织工程皮肤。

Abstract: The human amniotic membrane, type Ⅰ collagen were used as the scaffold, and the hair follicle stem cells and dermal cells from goat were used as the seed cells to construct the tissue engineered skin which would be have skin appendages such as hair follicles and sweat glands. Tissue engineered skin was constructed in vitro, and then was transplanted into goats to observe and test its repairing effect on damaged skin(as experimental group,and the transplantation of amniotic membrane instead of the tissue engineered skin was set as control). The results showed that hair follicle was formed when the tissue engineered skin was cultured for 25 days in vitro and the epidermal cells were keratinized by the scanning electron microscopy. The scar area was measured at 30, 60, and 90 days after transplantation. Results showed that the scar area in the experimental group was significantly lower than the control (P<0.01). When transplanted for 270 days, histological section of the transplanted skin showed that the dermal, epidermal and the hair follicles formed in the experiment group, and the control group were just connective tissues. In conclusion, the tissue engineering skin with hair follicles was successfully constructed using goat hair follicle stem cells and dermal cells as seed cells.

中图分类号:

Q813
R622.1

史明艳, 高雪, 杨学义, 窦忠英, 许尚忠. 构建具有毛囊结构的组织工程皮肤及移植研究[J]. 畜牧兽医学报, 2019, 50(10): 2097-2104.

SHI Mingyan, GAO Xue, YANG Xueyi, DOU Zhongying, XU Shangzhong. Construction and Transplantation of Tissue Engineered Skin with Hair Follicles[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2097-2104.

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构建具有毛囊结构的组织工程皮肤及移植研究

Construction and Transplantation of Tissue Engineered Skin with Hair Follicles

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