代乳粉添加枯草芽孢杆菌对7~28日龄湖羊羔羊胃肠道发育的影响

doi:10.11843/j.issn.0366-6964.2019.10.010

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (10): 2041-2052.doi: 10.11843/j.issn.0366-6964.2019.10.010

代乳粉添加枯草芽孢杆菌对7~28日龄湖羊羔羊胃肠道发育的影响

郑琛¹, 李发弟^2,3, 李飞², 周巨旺¹, 段鹏伟¹, 刘绘汇¹, 樊海苗¹, 朱威力¹, 刘婷^1*

1. 甘肃农业大学动物科学技术学院, 兰州 730070;
2. 兰州大学草地农业科技学院草地农业生态系统国家重点实验室, 农业农村部草牧业创新重点实验室, 兰州 730020;
3. 甘肃省肉羊繁育生物技术工程实验室, 民勤 733300

收稿日期:2019-04-18 出版日期:2019-10-23 发布日期:2019-10-23
通讯作者: 刘婷,主要从事反刍动物营养研究,E-mail:liuting@gsau.edu.cn
作者简介:郑琛(1978-),男,陕西佳县人,副教授,博士,主要从事反刍动物营养研究,Tel:0931-7631225,E-mail:zhengc@gsau.edu.cn
基金资助:
甘肃农业大学青年研究生指导教师扶持基金项目（GAU-QNDS-201603）

Effects of Bacillus Subtilis Supplementation into Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Day-old Hu Lambs

ZHENG Chen¹, LI Fadi^2,3, LI Fei², ZHOU Juwang¹, DUAN Pengwei¹, LIU Huihui¹, FAN Haimiao¹, ZHU Weili¹, LIU Ting^1*

1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation of Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
3. Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China

Received:2019-04-18 Online:2019-10-23 Published:2019-10-23

摘要/Abstract

摘要： 旨在观察代乳粉中添加枯草芽孢杆菌（Bacillus Subtilis）对7~28日龄湖羊羔羊胃肠道生长发育的影响。本研究选择健康、生长发育正常的7日龄湖羊公羔（双羔）30只，按同质性原则随机分为2组，每组15只，每只为1个重复，分别饲喂枯草芽孢杆菌含量为0和0.2%的代乳粉，试验期21 d。28日龄时每组随机选择8只羔羊进行屠宰，称量各胃室和各肠段内容物重量和净重量，量取各肠段长度，计算各段的相对重量和相对长度及内容物分布。采集皱胃胃底腺区及十二指肠、空肠和回肠中段的组织样品，用多聚甲醛固定后用于组织形态学测定，并测定小肠上皮细胞凋亡率。同时采集十二指肠、空肠和回肠黏膜，测定闭锁蛋白（occludin）、闭锁小带1（zonula occludens-1，ZO-1）和紧密连接蛋白1（claudin 1）mRNA表达量。结果表明：1）枯草芽孢杆菌显著降低7~28日龄湖羊羔羊十二指肠指数（占活体重量百分比，P=0.037）、空肠相对长度（占全肠长度百分比，P=0.009）和十二指肠绒毛宽度（P=0.001），显著升高羔羊结肠相对长度（占全肠长度百分比，P=0.012）、空肠内容物相对重量（占全肠内容物重量百分比，P=0.003）、回肠绒毛高度（P=0.010）和隐窝深度（P=0.034）；2）枯草芽孢杆菌显著上调十二指肠黏膜ZO-1蛋白mRNA表达量（P=0.012），此外，枯草芽孢杆菌有上调羔羊回肠黏膜occludin蛋白mRNA表达量以及降低回肠上皮细胞凋亡率的趋势（P=0.053，P=0.079）；3）枯草芽孢杆菌对其他胃肠段的相对重量（占活体重量、总胃重量、总肠重量和总胃肠重量百分比）及其内容物相对重量（占活体重量、总胃内容物重量、总肠内容物重量和总胃肠内容物重量百分比）、肠道相对长度（占全肠长度百分比）以及皱胃和小肠组织形态与小肠黏膜occludin蛋白、ZO-1蛋白和claudin 1蛋白mRNA表达量和小肠其他肠段上皮细胞凋亡率均未产生显著影响（P>0.05）。枯草芽孢杆菌对7~28日龄湖羊羔羊胃肠道生长发育基本无显著影响，但可提高小肠屏障功能并减少上皮细胞凋亡，有利于保持小肠结构和功能完整。

Abstract: The purpose of this study was to investigate the effects of Bacillus Subtilis supplementation into milk replacer on the development of gastrointestinal tract of 7-28 day-old Hu lambs. Thirty 7 day-old Hu male lambs(double lambs) were chosen and divided into 2 groups randomly, fifteen lambs in each group and each lamb as a repeat. Lambs in the 2 groups were fed milk replacer with 0 and 0.2% Bacillus Subtilis, respectively. The test lasted for 21 days. Eight lambs were selected from each group randomly and slaughtered at 28 day-old. The weight and net weight of content in the each stomach chamber and each intestinal segment, the length of the intestinal tracts were measured, and the relative weight, relative length and content distribution were calculated. The tissue samples from fundus gland region of the abomasum, the middle part of duodenum, jejunum and ileum were fixed in paraformaldehyde to analyse the histomorphology, and the apoptotic rate of small intestinal epithelial cells was detected. And the mRNA expression of occludin, ZO-1 and claudin 1 in duodenum, jejunum and ileum mucosa were measured. The results showed that:1) Bacillus Subtilis significantly decreased the duodenum index (percentage to body weight, P=0.037), relative length of jejunum (percentage to intestinal tract length, P=0.009) and villus width of duodenum of 7-28 day-old Hu lambs (P=0.001), and increased relative length of colon (percentage to intestinal tract length, P=0.012), relative weight of jejunum content (percentage to intestinal tract content weight, P=0.003), villus height and crypt depth of ileum (P=0.010, P=0.034); 2) Bacillus Subtilis significantly increased the mRNA expression of ZO-1 protein in duodenum mucosa of lambs (P=0.012), in addition, there was a tendency that Bacillus Subtilis up-regulated mRNA expression of oocludin protein in ileum mucosa (P=0.053) and reduced the apoptotic rate of ileum epithelial cells of lambs (P=0.079); 3) Bacillus Subtilis did not affect the relative weights of stomach,intestine segments (percentage to body weight, percentage to stomach weight, percentage to intestinal tract weight and percentage to gastrointestinal tract weight), relative weights of content of stomach and intestinal tract (percentage to body weight, percentage to stomach content weight, percentage to intestinal tract content weight and percentage to gastrointestinal tract content weight), gut relative lengths (percentage to intestinal tract length), histomorphology of abomasum and intestinal tract, mRNA expression of occludin, ZO-1 and claudin 1 proteins in intestine mucosa, and epithelial cells apoptosis rate of other parts of small intestine (P>0.05). It indicates that Bacillus Subtilis almost does not significantly influence the gastrointestinal tract developments of 7-28 day-old Hu lambs, but improves the barrier function of small intestine and decreases the apoptotic rate of small intestinal epithelial cells, beneficial for maintaining normal structure and function of intestinal tract.

中图分类号:

S826.5

郑琛, 李发弟, 李飞, 周巨旺, 段鹏伟, 刘绘汇, 樊海苗, 朱威力, 刘婷. 代乳粉添加枯草芽孢杆菌对7~28日龄湖羊羔羊胃肠道发育的影响[J]. 畜牧兽医学报, 2019, 50(10): 2041-2052.

ZHENG Chen, LI Fadi, LI Fei, ZHOU Juwang, DUAN Pengwei, LIU Huihui, FAN Haimiao, ZHU Weili, LIU Ting. Effects of Bacillus Subtilis Supplementation into Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Day-old Hu Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2041-2052.

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代乳粉添加枯草芽孢杆菌对7~28日龄湖羊羔羊胃肠道发育的影响

Effects of Bacillus Subtilis Supplementation into Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Day-old Hu Lambs

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