不同被毛密度獭兔皮肤差异表达基因的研究

doi:10.11843/j.issn.0366-6964.2014.10.008

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (10): 1631-1639.doi: 10.11843/j.issn.0366-6964.2014.10.008

不同被毛密度獭兔皮肤差异表达基因的研究

李冰，李福昌^*，朱岩丽，王雪鹏，王春阳

（山东农业大学动物科技学院，泰安 271018）

收稿日期:2014-03-03 出版日期:2014-10-23 发布日期:2014-10-23
通讯作者: 李福昌，E-mail：chlf@sdau.edu.cn
作者简介:李冰（1987-），男，山东青岛人，硕士生，主要从事动物营养和家兔生产研究，Tel：0538-8249222-8319，E-mail：libing881011@163.com
基金资助:
现代农业产业技术体系建设专项（CARS-44-B-1）；国家公益性行业（农业）科研专项（200903006）

Detection of Differentially Expressed Genes in Rex Rabbit Skin with Different Wool Density

LI Bing，LI Fu-chang^*，ZHU Yan-li，WANG Xue-peng，WANG Chun-yang

(College of Animal Science and Technology，Shandong Agricultural University,Tai’an 271018,China)

Received:2014-03-03 Online:2014-10-23 Published:2014-10-23

摘要/Abstract

摘要：

本研究旨在筛选4日龄不同被毛密度獭兔皮肤的差异表达基因。采用Agilent公司家兔全基因表达谱芯片对4日龄不同被毛密度獭兔皮肤组织进行差异基因筛选，并通过实时荧光定量PCR方法对部分差异显著基因进行验证。试验共筛选出188个差异基因，与低被毛密度獭兔相比，高被毛密度獭兔皮中表达上调基因72个，其中包含24个已知功能基因，表达下调基因116个，其中包含38个已知功能基因。GO（Gene ontology）分类显示，大部分差异基因参与细胞增殖、凋亡、氧化还原、脂代谢分解等生命过程。KEGG（Kyoto encyclopedia of genes and genomes）通路分析显示，差异基因主要参与Wnt信号通路、PPAR信号通路、维生素合成与代谢途径等。荧光定量PCR结果与基因芯片结果基本吻合，证实基因芯片结果可靠。结果表明，差异倍数较大的差异基因，像胰岛素样生长因子（IGF-I）、血管内皮生长因子（VEGF）和骨形态发生蛋白（BMP2）可能与獭兔被毛的形成有关；Wnt信号通路、PPAR信号通路等，以及维生素合成与代谢途径等也可能参与被毛的发育过程。

Abstract:

In order to screen different expressed genes for 4-day-old rex rabbit with different wool density.The Agilent’s rabbit gene expression microarray was used to determine the differentially expressed genes.The expression patterns of selected differentially expressed genes were further indentified by quantitative real-time PCR.The results showed that a total of 188 differentially expressed genes associated with rex rabbit wool density were screened out.These genes included 72 up-regulated genes which contained 24 known function genes，and 116 down-regulated genes which contained 38 known function genes.GO analysis results revealed that the expression levels of most differentially expressed genes were related to cell proliferation，apoptosis，oxidation-reduction，lipoprotein metabolic ect.KEGG pathway analysis revealed that these differential genes were mainly involved in the signaling pathways of Wnt and PPAR，and vitamin synthesis and metabolism.In addition，the results of qRT-PCR were consistent with the results of microarray hybridization，indicating that the data collected from microarray hybridization were reliable.The results suggest that the genes，such as insulin-like growth factor I (IGF-I)，vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP2)，may be related to the formation of wool in rex rabbit skin.And the pathway Wnt，PPAR，synthetize and metabolism of vitamin might be participate in the process of wool development in rex rabbit skin.

中图分类号:

李冰，李福昌，朱岩丽，王雪鹏，王春阳. 不同被毛密度獭兔皮肤差异表达基因的研究[J]. 畜牧兽医学报, 2014, 45(10): 1631-1639.

LI Bing，LI Fu-chang，ZHU Yan-li，WANG Xue-peng，WANG Chun-yang. Detection of Differentially Expressed Genes in Rex Rabbit Skin with Different Wool Density[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1631-1639.

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不同被毛密度獭兔皮肤差异表达基因的研究

Detection of Differentially Expressed Genes in Rex Rabbit Skin with Different Wool Density

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