日粮尿素水平对肉羊血液学、血清学指标和消化器官的影响

doi:10.11843/j.issn.0366-6964.2014.09.010

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (9): 1449-1456.doi: 10.11843/j.issn.0366-6964.2014.09.010

日粮尿素水平对肉羊血液学、血清学指标和消化器官的影响

王波¹，姜成钢¹,纪守坤¹,王黎文²,丁健²,司丙文¹，屠焰¹，张乃锋¹，刁其玉^1*

（1.中国农业科学院饲料研究所/农业部饲料生物技术重点实验室，北京100081；2.全国畜牧兽医总站，北京 100026）

收稿日期:2014-03-16 出版日期:2014-09-23 发布日期:2014-09-23
通讯作者: 刁其玉，研究员，博士生导师，E-mail：diaoqiyu@caas.cn
作者简介:王波（1989-），男，河南新县人，硕士生，主要从事动物营养与饲料科学研究，E-mail：wangboforehead@163.com；姜成钢（1980-），男，浙江衢州人，博士生，主要从事动物营养与饲料科学研究，E-mail:cgj027@163.com
基金资助:
农产品质量安全专项资金（2013）；牧区饲草饲料资源开发利用技术研究与示范（20120304202）

Effects of Different Levels of Urea in Diets on Haematological Indices，Serological Parameters and Tissue Lesions in Mutton Sheep

WANG Bo¹，JIANG Cheng-gang¹，JI Shou-kun¹，WANG Li-wen²，DING Jian²，SI Bing-wen¹，TU Yan¹，ZHANG Nai-feng¹，DIAO Qi-yu^1*

(1.Key Laboratory of Feed Biotechnology of the Ministry of Agriculture，Feed Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2.Station of Animal and Veterinary of China，Beijing 100026，China）

Received:2014-03-16 Online:2014-09-23 Published:2014-09-23

摘要/Abstract

摘要：

本试验旨在研究肉羊日粮中添加不同水平尿素替代天然蛋白质饲料对血液学、血清学指标及消化器官组织病理学的影响，为尿素的安全使用提供依据。试验选取杜寒杂交公羊64只，平均体重为（30.77±0.02）kg，随机分4个处理：对照组、0.5%尿素组、1.5%尿素组和2.5%尿素组。预试期21 d，正式期42 d，分别在试验的第1和第42天处理8只羊采血备用，试验结束后进行屠宰，取十二指肠、瘤胃、肝脏、肾脏等组织进行病理学检查。血液学结果表明：1.5%和2.5%组的血小板数显著高于对照组和0.5%组（P<0.05）；平均血红蛋白浓度2.5%组显著高于0.5%组（P<0.05）；2.5%组的平均红细胞血红蛋白含量显著高于其他3个试验组（P<0.05）。血清生化指标结果表明：1.5%和2.5%组血清尿素氮水平显著低于对照组和0.5%组（P<0.05），甘油三酯则1.5%和2.5%组显著高于对照组和0.5%组（P<0.05），2.5%组的谷丙转氨酶、谷草转氨酶、肌酐、总胆红素及血氨浓度均最高（P<0.05），2.5%组的碱性磷酸酶浓度显著低于对照组（P<0.05），各组其余血清生化指标差异不显著（P>0.05）。通过组织切片病理学检查发现2.5%组的肾病变严重性较其余3组差异显著（P<0.05）。结果提示，日粮中1.5%水平以下尿素含量对肉羊血液学、血清学及主要消化器官影响不明显，而尿素水平达到2.5%则会对肉羊健康造成严重影响，存在安全隐患。

Abstract:

This experiment aimed to study the effects of substitution of different levels of urea for natural protein on haematological indices，serological parameters and the histopathology of digestive organs in mutton sheep and provide basis for the safe use of urea.Sixty four Dorper×thin-tailed Han crossbred male lambs with average body weight of (30.77±0.02)kg were randomly divided into 4 treatments as follows：control group and control group supplemented with 0.5%，1.5%，and 2.5% urea,respectively.The investigation lasted for 63 days including 21 days of adaptation.Each treatment including 8 sheep and blood was sampled on d 1 and d 42.Thirty two lambs were slaughtered at the end of the experiment，and the sample of duodenum，rumen，liver and kidney were taken for pathological examination.The detection results of haematological indices showed that the platelet count of 1.5% and 2.5% urea group were significant higher than that of the control group and 0.5% urea group (P<0.05)；the mean corpuscular hemoglobin concentration of 2.5% urea group was also remarkably higher than that of 0.5% urea group (P<0.05)；average red blood cell hemoglobin content of 2.5% urea group was higher than that of other 3 groups (P<0.05).Blood urea nitrogen of 1.5% and 2.5% urea groups were significant lower than that of control and 0.5% urea group (P<0.05).However，the effect of urea supplementation on triacylglycerol was opposite to the results of blood urea nitrogen (P<0.05)；the concentration of glutamate pyruvate transaminase，glutamic-oxaloacetic transaminase，creatinine total bilirubin and blood ammonia in 2.5% urea group were the highest among the 4 groups (P<0.05)，but the concentration of alkaline phosphatase of 2.5% group was lower than that of control group (P<0.05)；the rest indices of serological parameters were not remarkably different among different groups (P>0.05).The lesion of kidney of 2.5% urea group was more serious than that of other groups (P<0.05).These results indicated that，the haematological indices，serological parameters and the main digestive organs of sheep were not significantly affected when the level of urea in ration under 1.5%.However，the health of mutton sheep will be seriously harmed as the supplementation of urea increased to 2.5% in feed.

中图分类号:

S826
S816.32

王波，姜成钢,纪守坤,王黎文,丁健,司丙文，屠焰，张乃锋，刁其玉. 日粮尿素水平对肉羊血液学、血清学指标和消化器官的影响[J]. 畜牧兽医学报, 2014, 45(9): 1449-1456.

WANG Bo，JIANG Cheng-gang，JI Shou-kun，WANG Li-wen，DING Jian，SI Bing-wen，TU Yan，ZHANG Nai-feng，DIAO Qi-yu. Effects of Different Levels of Urea in Diets on Haematological Indices，Serological Parameters and Tissue Lesions in Mutton Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1449-1456.

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日粮尿素水平对肉羊血液学、血清学指标和消化器官的影响

Effects of Different Levels of Urea in Diets on Haematological Indices，Serological Parameters and Tissue Lesions in Mutton Sheep

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