ST9型动物源耐甲氧西林金黄色葡萄球菌的分子分型、毒素基因检测及耐药性分析

doi:2013-11-11

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (5): 853-858.doi: 2013-11-11

• 研究简报 • 上一篇

ST9型动物源耐甲氧西林金黄色葡萄球菌的分子分型、毒素基因检测及耐药性分析

张强¹，邢晓楠¹，王新^1*，王晓²，俞英²，吴聪明³，沈建忠³

（1.西北农林科技大学食品科学与工程学院，杨凌 712100；2.中国农业大学动物科技学院，北京 100193；3.中国农业大学动物医学院，北京 100193）

收稿日期:2013-11-11 出版日期:2014-05-23 发布日期:2014-05-23
通讯作者: 王新（1973-），男，四川邛崃人，副教授，主要从事食源性病原及分子生物学和食品安全研究，E-mail：xinwang7516@nwsuaf.edu.cn
作者简介:张强（1974-），男，陕西富平人，实验师，主要从事食品质量安全（微生物）检测研究，E-mail：zhqiang001@nwsuaf.deu.cn
基金资助:
国家自然科学基金（31271858）；中央高校基本科研业务费专项资金（QN2011139）

Antimicrobial Susceptibility，Virulence Factors and Molecular Typing of Methicillin-resistant Staphylococcus aureus ST9 from Dairy Cow and Swine

ZHANG Qiang¹， XING Xiao-nan¹， WANG Xin^1*， WANG Xiao²， YU Ying²， WU Cong-ming³， SHEN Jian-zhong³

(1.College of Food Science and Engineering， Northwest A & F University， Yangling 712100， China;2.College of Animal Science and Technology， China Agricultural University， Beijing 100193， China;3.Department of Pharmacology & Toxicology， College of Veterinary Medicine，
China Agricultural University， Beijing 100193， China)

Received:2013-11-11 Online:2014-05-23 Published:2014-05-23

摘要/Abstract

摘要：

为了解动物源耐甲氧西林金黄色葡萄球菌（MRSA）ST9型的耐药表型、毒素基因和分子型，采用琼脂平板稀释法、药敏纸片法、多位点序列分型(multilocus sequence typing，MLST)、SCCmec分型、spa分型和PFGE分型技术分别对12株猪源和6株牛源 MRSA ST9进行耐药性、毒素基因和分子分型研究。结果显示这些MRSA ST9型菌株除了对万古霉素100%敏感外，对其余31种抗生素均产生了不同程度的耐药，其耐药率在5.6％~100％；所有菌株(100%)均携带有毒素基因，毒素基因的检测依次为seg（94.4%）、seb（83.3%）、sed（72.2%）、PVL（55.6%）、sei（11.1%）和sea（5.6%），均未检测到ETs、tsst-1、sec、see、seh和sej基因，得到10个毒力基因型；分子型以ST9-SCCmec IVb-t899克隆系为主（17株，94.4%），其次是MRSA-ST9-SCCmec II-t899（1株，5.6%）。PFGE分型证实MRSA ST9存在一定的传播性。本研究MRSA ST9型菌株主要流行ST9-SCCmec IVb-t899克隆系，该克隆系携带较多毒素基因、多重耐药和传播性，给动物和人类健康构成潜在的风险。

Abstract:

The aim of this study was to investigate antimicrobial resistance and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) ST 9 from dairy herds and swine.MRSA ST9 isolates were characterized by antimicrobial susceptibility test，pulsed-field gel electrophoresis (PFGE)，spa typing，and SCCmec typing，and were examined by PCR for genes encoding enterotoxins，exfoliative toxins，Panton-Valentine leukocidin (PVL)，and toxic shock syndrome toxin 1 (Tsst-1).All MRSA ST9 isolates were sensitive to vancomycin.But they showed resistance against other 31 antibiotics to various degrees ranging from 5.6% to 100%.In addition，100% of isolates were positive for one or more toxin genes tested.The four most predominant toxin genes were seg (94.4%)，seb (83.3%)，sed (72.2%) and PVL (55.6%)，followed by sei (11.1%) and，sea (5.6%).ETs，Tsst-1, sec，see，seh and sej genes were not detected.MRSA ST9 isolates belonged to ST9-SCCmec IVb-t899 (94.4%) and ST9-SCCmec II-t899 (5.6%).PFGE suggested potential transmission of MRSA ST9 strains in different farms.Many MRSA ST9-SCCmec IVb-t899 isolates harbored multiple toxin genes and exhibited multiple antimicrobial resistance.The presence of MRSA ST9 strains in these animals poses a potential threat to animal and human health.

中图分类号:

R378.11

张强，邢晓楠，王新，王晓，俞英，吴聪明，沈建忠. ST9型动物源耐甲氧西林金黄色葡萄球菌的分子分型、毒素基因检测及耐药性分析[J]. 畜牧兽医学报, 2014, 45(5): 853-858.

ZHANG Qiang， XING Xiao-nan， WANG Xin， WANG Xiao， YU Ying， WU Cong-ming， SHEN Jian-zhong. Antimicrobial Susceptibility，Virulence Factors and Molecular Typing of Methicillin-resistant Staphylococcus aureus ST9 from Dairy Cow and Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 853-858.

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ST9型动物源耐甲氧西林金黄色葡萄球菌的分子分型、毒素基因检测及耐药性分析

Antimicrobial Susceptibility，Virulence Factors and Molecular Typing of Methicillin-resistant Staphylococcus aureus ST9 from Dairy Cow and Swine

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