鸭骨骼肌早期发育过程中钙蛋白酶3（CAPN3）基因的表达及其与肌纤维性状的关联

doi:10.11843/j.issn.0366-6964.2014.03.007

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (3): 385-390.doi: 10.11843/j.issn.0366-6964.2014.03.007

鸭骨骼肌早期发育过程中钙蛋白酶3（CAPN3）基因的表达及其与肌纤维性状的关联

朱文奇^1，2，徐文娟²，束婧婷²，陈文峰³，单艳菊²，刘宏祥²，胡艳²，李慧芳^2*，王金玉^1*

（1.扬州大学动物科学与技术学院，扬州 225009； 2.江苏省家禽科学研究所，扬州 225125；3.江苏腾达源农牧有限公司，姜堰 225538）

收稿日期:2013-08-27 出版日期:2014-03-23 发布日期:2014-03-23
通讯作者: 李慧芳，研究员，博士，主要从事家禽遗传育种研究，Tel：0514-85599266，E-mail：lhfxf_002@yahoo.com.cn；王金玉，教授，主要从事动物遗传育种研究，Tel：0514-87979075，E-mail：jywang@yzu.edu.cn
作者简介:朱文奇（1981-），男，助理研究员，博士生，主要从事家禽遗传育种研究，Tel：0514-85599179，E-mail：zwq.js@126.com；徐文娟（1980-），女，助理研究员，硕士，主要从事家禽分子育种研究，Tel：0514-85599179，E-mail：47691776@qq.com。二者并列为第一作者
基金资助:
国家自然科学基金（31172194）；江苏省科技支撑计划项目（BE2011329）；现代农业品种创新项目（CX(11)1030）

Expression of CAPN3 Gene in Skeletal Muscles and Its Association with Myofiber Traits during Embryonic and Early Post-hatching Development in Ducks

ZHU Wen-qi^1,2，XU Wen-juan²，SHU Jing-ting²，CHEN Wen-feng³，SHAN Yan-ju²，LIU Hong-xiang²，HU Yan²，LI Hui-fang^2*，WANG Jin-yu^1*

（1.College of Animal Science and Technology，Yangzhou University，Yangzhou 225009，China;2. Jiangsu Institute of Poultry Science，Yangzhou 225125，China； 3. Jiangsu Tengdayuan Co.Ltd, Jiangyan 225538，China)

Received:2013-08-27 Online:2014-03-23 Published:2014-03-23

摘要/Abstract

摘要：

本研究选择生长速度不同的高邮鸭和金定鸭为试验材料，对不同品种鸭胚胎期和出雏早期肌肉中钙蛋白酶3(calpain3，CAPN3)基因的表达及其与肌纤维性状的关联性进行了研究。采用实时荧光定量PCR方法研究鸭13、17、21、25、27胚龄和出雏后7日龄胸肌和腿肌中CAPN3基因表达的发育性变化。结果发现，CAPN3 mRNA在2个品种鸭早期肌肉发育中具有相似的表达模式，并表现出显著的时间特异性，而品种和性别效应并不显著。在胸肌中，呈现先下降后上升的趋势，25胚龄前表达量均较低，21胚龄最低，出雏前（27胚龄）至7日龄表达量显著上升。在腿肌中，呈现“波浪形”表达模式，在21胚龄表达量最高。腿肌CAPN3基因表达与肌纤维类型、直径、面积和密度之间呈现不同程度的线性相关，部分指标在金定鸭中达到了显著水平。初步推测，CAPN3基因与肌纤维类型和肌纤维的生长发育密切相关。

Abstract:

In the present study，expression of CAPN3 gene was quantified by RT-PCR in the breast muscle and leg muscle tissues on days 13(E13 d)，17(E17 d)，21(E21 d)，25(E25 d)，27(E27 d) of embryonic development，as well as at 7 days post-hatching (7 d PH) in Gaoyou and Jinding ducks with different growth rates.Meanwhile，the association between expression level of CAPN3 gene and myofiber traits was also analyzed. The results showed that the CAPN3 mRNA expression profile had a similar trend in both breeds and showed an extremely significant specific expression at different development stages，breeds and gender had no effect on its expression.In breast muscle，the expression level of CAPN3 mRNA was relatively low before E25 d and the lowest point appeared on E21 d，then increased significantly before hatching (E27 d) and 7 d PH.In leg muscle，the expression profile of CAPN3 showed a “wave” trend，the expression peaking appeared on E21 d.Meanwhile different linear correlations were found between CAPN3 gene mRNA expression and myofiber types，diameter，cross-sectional area and density in duck.In Jinding ducks，CAPN3 gene mRNA expression was significantly correlated with myofiber diameter，cross-sectional area and density.These results suggest that CAPN3 may have potential functions in controlling muscle fiber phenotype and development during embryonic and early post-hatching development in ducks.

中图分类号:

S834
S813.3

朱文奇，徐文娟，束婧婷，陈文峰，单艳菊，刘宏祥，胡艳，李慧芳，王金玉. 鸭骨骼肌早期发育过程中钙蛋白酶3（CAPN3）基因的表达及其与肌纤维性状的关联[J]. 畜牧兽医学报, 2014, 45(3): 385-390.

ZHU Wen-qi，XU Wen-juan，SHU Jing-ting，CHEN Wen-feng，SHAN Yan-ju，LIU Hong-xiang，HU Yan，LI Hui-fang，WANG Jin-yu. Expression of CAPN3 Gene in Skeletal Muscles and Its Association with Myofiber Traits during Embryonic and Early Post-hatching Development in Ducks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 385-390.

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鸭骨骼肌早期发育过程中钙蛋白酶3（CAPN3）基因的表达及其与肌纤维性状的关联

Expression of CAPN3 Gene in Skeletal Muscles and Its Association with Myofiber Traits during Embryonic and Early Post-hatching Development in Ducks

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