小鼠早期胚胎发育过程中的DNA去甲基化

摘要/Abstract

摘要：

表观遗传修饰在基因转录与表达、细胞生长与分化以及动物个体正常发育等过程中都具有重要的调控作用。表观遗传修饰发生异常，会引起机体生长发育中的各种异常。哺乳动物从精卵受精到附植前的胚胎早期发育阶段会发生重要的表观遗传重编程，主要包括DNA甲基化和组蛋白修饰。精卵受精后DNA发生主动和被动2种方式的去甲基化。本文主要综述了与DNA甲基化相关的蛋白和早期胚胎发育过程中的去甲基化机制，并对小鼠附植前胚胎发育过程中的DNA甲基化的动态变化进行了详细的论述。

Abstract:

Epigenetic modifications play essential regulatory roles in gene expression and transcription, cell growth &differentiation, and growth of animal. The abnormality of epigenetic modification can lead to a series of disease during development. From fertilization to implantation, epigenome of embryos undergoes extensive reprogramming, which mainly induce demethylation of 5mC and histone modifications. After fertilization, genome undergoes both active and passive demethylation in mammalian pre-implantation embryos. This paper summarizes proteins related to DNA methlation modification and mechanisms of DNA demethylation, and the dynamic changes of DNA methylation in mouse pre-implantation embryos.

中图分类号:

刘青青，郑丽明，刘红亮，苏建民，权富生，张涌. 小鼠早期胚胎发育过程中的DNA去甲基化[J]. 畜牧兽医学报, doi: .

LIU Qing-qing, ZHENG Li-ming, LIU Hong-liang, SU Jian-min, QUAN Fu-sheng, ZHANG Yong. DNA Demethylation in Mouse Pre-implantation Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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参考文献

［1］BIRD A. Perceptions of epigenetics［J］. Nature, 2007, 447(7143): 396-398.

［2］BONASIO R, TU S, REINBERG D. Molecular signals of epigenetic states［J］. Science, 2010, 330(6004): 612-616.

［3］BERNSTEIN B E, MEISSNER A, LANDER E S. The mammalian epigenome［J］. Cell, 2007, 128(4): 669-681.

［4］SURANI M A, HAYASHI K, HAJKOVA P. Genetic and epigenetic regulators of pluripotency［J］. Cell, 2007, 128: 747-762.

［5］BESTOR T, LAUDANO A, MATTALIANO R, et al. Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases［J］. J Mol Biol, 1988, 203(4): 971-983.

［6］LEI H, OH S P, OKANO M, et al. De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells［J］. Development, 1996, 122(10): 3195-3205.

［7］GOLL M G, KIRPEKAN F, MAGGERT K A, et al. Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2［J］. Science, 2006, 311(5759): 395-398.

［8］OKANO M, XIE S, LI E. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells［J］. Nucleic Acids Res, 1998, 26(11): 2536-2540.

［9］KANEDA M, OKANO M, HATA K, et al. Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting［J］. Nature, 2004, 429(6994): 900-903.

［10］OKANO M, BELL D W, HABER D A, et al. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development［J］. Cell, 1999, 99(3): 247-257.

［11］UEDA Y, OKANO M, WILLIAMS C, et al. Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome［J］. Development, 2006, 133(6): 1183-1192.

［12］BOURC'HIS D, BESTOR T H. Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3L［J］. Nature, 2004, 431(7004): 96-99.

［13］BOURC'HIS D, XU G L, LIN C S, et al. Dnmt3L and the establishment of maternal genomic imprints［J］. Science, 2001, 294(5551): 2536-2539.

［14］HATA K, OKANO M, LEI H, et al. Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice［J］. Development, 2002, 129(8): 1983-1993.

［15］WEBSTER K E, O'BRYAN M K, FLETCHER S, et al. Meiotic and epigenetic defects in Dnmt3L-knockout mouse spermatogenesis［J］. Proc Natl Acad Sci USA, 2005, 102(11): 4068-4073.

［16］BOSTICK M, KIM J K, ESTEVE P O, et al. UHRF1 plays a role in maintaining DNA methylation in mammalian cells［J］. Science, 2007, 317(5845): 1760-1764.

［17］FUJIMORI A, MATSUDA Y, TAKEMOTO Y, et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation［J］. Mamm Genome, 1998, 9(12): 1032-1035.

［18］SHARIF J, MUTO M, TAKEBAYASHI S, et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA［J］. Nature, 2007, 450(7171): 908-912.

［19］DAWALAT M M, GANZ K, PIWELL B E, et al. Tet1 is dispensable for maintaining pluripotency and its loss is compatible with embryonic and postnatal development［J］. Cell Stem Cell, 2011, 9(2): 166-175.

［20］ITO S, D'ALESSIO A C, TARANOVA O V, et al. Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification［J］. Nature, 2010, 466(7310): 1129-1133.

［21］SZWAGIERCZAK A, BULTMANN S, SCHMIDT C S, et al. Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA［J］. Nucleic Acids Res, 2010, 38(19): e181.

［22］TAHILIANI M, KOH K P, SHEN Y, et al. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1［J］. Science, 2009,324(5929): 930-935.

［23］FIGUEROA M E, ABDEL-WAHAB O, LU C, et al. Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation［J］. Cancer Cell, 2010, 18(6): 553-567.

［24］MORAN-CRUSIO K, REAVIE L, SHIH A, et al. Tet2 loss leads to increased hematopoietic stem cell self-renewal and myeloid transformation［J］. Cancer Cell, 2011, 20(1): 11-24.

［25］QUIVORON C, COURONNE L, DELLA VALLE V, et al. TET2 inactivation results in pleiotropic hematopoietic abnormalities in mouse and is a recurrent event during human lymphomagenesis［J］. Cancer Cell, 2011, 20(1): 25-38.

［26］GU T P, GUO F, YANG H, et al. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes［J］. Nature, 2011, 477(7366): 606-610.

［27］WOSSIDLO M, NAKAMURA T, LEPIKHOV K, et al. 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming［J］. Nat Commun, 2011, 2: 241.

［28］MURAMATSU M, SANKARANAND V S, ANANT S, et al. Specific expression of activationinduced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells［J］. J Biol Chem, 1999, 274(26): 18470-18476.

［29］MURAMATSU M, KINOSHITA K, FAGARASANS, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme［J］. Cell, 2000, 102(5): 553-563.

［30］GUO J U, SU Y, ZHONG C, et al. Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain［J］. Cell, 2011, 145(3): 423-434.

［31］HIRANO K, YOUNG S G, FARESE R V, et al. Targeted disruption of the mouse apobec-1 gene abolishes apolipoprotein B mRNA editing and eliminates apolipoprotein B48［J］. J Biol Chem, 1996, 271(17): 9887-9890.

［32］HAJKOVA P, GEFFRIES S J, LEE C, et al. Genome-wide reprogramming in the mouse germ line entails the base excision repair pathway［J］. Science, 2010, 329(5987): 78-82.

［33］HAJKOVA P, ANCELIN K, WALDMANN T, et al. Chromatin dynamics during epigenetic reprogramming in the mouse germ line［J］. Nature, 2008, 452(7189): 877-881.

［34］MAYER W, NIVELEAU A, WALTE R, et al. Demethylation of the zygotic paternal genome［J］. Nature, 2000, 403(6769): 501-502.

［35］NAKAMURA T, ARAI Y, UMEHARA H, et al. PGC7/Stella protects against DNA demethylation in early embryogenesis［J］. Nat Cell Biol, 2007, 9: 64-71.

［36］WOSSIDLO M, ARAND J, SEBASTIANO V, et al. Dynamic link of DNA demethylation, DNA strand breaks and repair in mouse zygotes［J］. EMBO J, 2010, 29(11): 1877-1888.

［37］LAW J A, JACOBSEN S E. Establishing, maintaining and modifying DNA methylation patterns in plants and animals［J］. Nat Rev Genet, 2010, 11(3): 204-220.

［38］ZIEGLER-BIRLING C, HELMRICH A, TORA L, et al. Distribution of p53 binding protein 1 (53BP1) and phosphorylated H2A.X during mouse preimplantation development in the absence of DNA damage［J］. Int J Dev Biol, 2009, 53(7): 1003-1011.

［39］ODA M, GLASS J L, THOMPSON R F, et al. High-resolution genome-wide cytosine methylation profiling with simultaneous copy number analysis and optimization for limited cell numbers［J］. Nucleic Acids Res, 2009, 37(12): 3829-3839.

［40］SMALLWOOD S A, TOMIZAWA S, KRUEGER F, et al. Dynamic CpG island methylation landscape in oocytes and preimplantation embryos［J］. Nat Genet, 2011, 43(8): 811-814.

［41］ZHU J K. Active DNA demethylation mediated by DNA glycosylases［J］. Annu Rev Genet, 2009, 43: 143-166.

［42］BRANCO M R, ODA M, REIK W. Safeguarding parental identity: Dnmt1 maintains imprints during epigenetic reprogramming in early embryogenesis［J］. Genes Dev, 2008, 22(12): 1567-1571.

［43］GEHRING M, BUBB K L, HENIKOFF S. Extensive demethylation of repetitive elements during seed development underlies gene imprinting［J］. Science, 2009, 324(5933): 1447-1451.

［44］HIRASAWA R, CHIBA H, KANEDA M, et al. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development［J］. Genes Dev, 2008, 22(12): 1607-1616.

［45］BORGEL J, GUIBERT S, LI Y, et al. Targets and dynamics of promoter DNA methylation during early mouse development［J］. Nat Genet, 2010, 42(12): 1093-1100.

［46］GAUDET F, RIDEOUT W M, 3RD MEISSNER A, et al. Dnmt1 expression in pre- and postimplantation embryogenesis and the maintenance of IAP silencing［J］. Mol Cell Biol, 2004, 24(4): 1640-1648.

［47］TOMIZAWA S, KOBAYASHI H, WATANABE T, et al. Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes［J］. Development, 2011, 138(5): 811-820.

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