σB因子在单核细胞增生李斯特菌环境应激反应中的调控作用

畜牧兽医学报

σ^B因子在单核细胞增生李斯特菌环境应激反应中的调控作用

杨丽红¹，张再超¹，孟庆玲^1,2*，乔军^1*，才学鹏²，蔡扩军¹，王为升¹，王俊伟¹，陈创夫¹

（1. 石河子大学新疆地方与民族高发病教育部重点实验室，石河子 832000；2. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室, 兰州 730046）

收稿日期:2012-09-20 出版日期:2013-03-23 发布日期:2013-03-23
通讯作者: 孟庆玲，E-mail: xjmqlqj@163.com,Tel：0993-2055036；乔军，E-mail: qj710625@yahoo.com.cn
作者简介:杨丽红（1984-），女，新疆五家渠人，硕士，主要从事病原分子生物学研究，E-mail:aybtor@163.com; 张再超（1987-），男，山东郓城人，硕士，主要从事病原分子生物学研究。杨丽红和张再超为并列第一作者

Regulating Role of σ^B Factor in the Environmental Stress Response of Listeriamonocytogenes

YANG Li-hong¹, ZHANG Zai-chao¹, MENG Qing-ling^1,2*, QIAO Jun^1*, CAI Xue-peng²,CAI Kuo-jun¹, WANG Wei-sheng¹, WANG Jun-wei¹, CHEN Chuang-fu¹

（1. Key Laboratory of Xinjiang Endemic and Ethnic Diseases of Ministry of Education, ShiheziUniversity, Shihezi 832000, China; 2. Key Laboratory of National Veterinary Etiological Biology,Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou730046, China)

Received:2012-09-20 Online:2013-03-23 Published:2013-03-23

摘要/Abstract

摘要：

为了解σ^B因子在单核细胞增生李斯特菌（LM）环境应激反应中的调控作用，笔者用基因重叠延伸PCR（SOE-PCR）和同源重组的方法构建了SigmaB基因缺失株，分析该缺失株在不同温度、pH和高盐压力条件下的应激反应能力及应激相关基因的转录水平。结果表明：与野毒株相比，SigmaB基因缺失株在压力条件下的应激能力减弱，rsbV、rsbW、hpt、clpP、ctsR等环境应激相关基因转录水平均降低。本研究提示σ^B因子在LM温度、pH和高盐环境应激反应中具有重要的调控作用，这为揭示LM环境应激反应的分子机制及药物作用新靶点的筛选提供了重要的理论依据。

Abstract:

This experiment was conducted to understand the role of the σ^Bfactor in regulating the environmental stress of Listeria monocytogenes (LM). We constructed SigmaB gene deletion LM strain by using gene overlap extension PCR (SOE-PCR) and homologous recombination, and the environmental stress response of the strain on the different temperature, different pH condition and high osmotic pressure conditions were detected, and the transcription level of the genes that related with environment stress were tested by using qRT-PCR. The results showed that the environmental stress abilities of the SigmaB gene deletion mutant at the non-suitable conditions weakened and the transcriptional level of genes corresponding to environmental stress (rsbV, rsbW, hpt, clpP, ctsR) decreased significantly comparing with the standard strain. The results indicated that σ^B factor plays an important role in responding to environmental stress (temperature, pH and osmotic pressure) of LM. The study provides an important theoretical basis for further studies on its molecular mechanisms of environmental stress response and new target for drug action.

中图分类号:

杨丽红，张再超，孟庆玲，乔军，才学鹏，蔡扩军，王为升，王俊伟，陈创夫. σ^B因子在单核细胞增生李斯特菌环境应激反应中的调控作用[J]. 畜牧兽医学报, doi: .

YANG Li-hong, ZHANG Zai-chao, MENG Qing-ling, QIAO Jun, CAI Xue-peng,CAI Kuo-jun, WANG Wei-sheng, WANG Jun-wei, CHEN Chuang-fu. Regulating Role of σ^B Factor in the Environmental Stress Response of Listeriamonocytogenes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2013/V44/I3/441

参考文献

［1］孟庆玲, 乔军, 才学鹏, 等. 单核细胞增多性李氏杆菌缺失株InlB的分子特征及其表达和纯化研究［J］. 微生物学报, 2007, 47(6): 1098-1101.

［2］乔军, 孟庆玲, 陈创夫, 等. 塔里木马鹿单核细胞增多性李氏杆菌的分离与鉴定［J］. 中国农学通报, 2008, 24(1)：9-12.

［3］ZHOU X, JIAO X, WIEDMANN M. Listeria monocytogenes in the Chinese food system: strain characterization through partial actA sequencing and tissue-culture pathogenicity assays［J］. J Med Microbiol, 2005, 54(3): 217224.

［4］FRASER K R, SUE D, WIEDMANN M, et al. Role of sigmaB in regulating the compatible solute uptake systems of Listeria monocytogenes osmotic induction of opuC is sigmaB dependent ［J］. Appl Environ Microbiol, 2003, 69(4): 2015-2022.

［5］冯莹颖, 张晓莉, 罗勤, 等. SignaB因子活性的调节及其在几种革兰阳性食源性致病菌中的作用［J］. 微生物学报, 2008, 48(6): 839-843.

［6］SUE D, FINK D, WIEDMANN M, et al. SigmaB-dependent gene induction and expression in Listeria monocytogenes during osmotic and acid stress conditions simulating the intestinal environment［J］. Microbiology, 2004, 150(11): 3843-3855.

［7］ADRIANA F, MICHAEL G, MARTIN W, et al. Comparative genomic analysis of the sigB operon in Listeria monocytogenes and in other gram-positive bacteria ［J］. Curr Microbiol, 2004, 48(1): 3946.

［8］SCOTT J M, MITCHELL T, HALDENWANG W G. Stress triggers a process that limits activation of the Bacillus subtilis stress transcription factor σ^B ［J］. J Bacteriol, 2000, 182: 1452-1456.

［9］ROBERTS A J, WIEDMAN N M. Pathogen, host and environmental factors contributing to the pathogenesis of listeriosis［J］. Cell Mol Life Sci, 2003, 60(5): 904-918.

［10］NADON C A, BOWEN B M, WIEDMANN M, et al. SigmaB contributes to PrfA-mediated virulence in Listeria monocytogenes［J］. Infect Immun, 2002, 70 (7): 3948-3952.

［11］KAZMIERCZAK M J, MITHOE S C, BOOR K J, et al. Listeria monocytogenes σ^B regulates stress response and virulence functions［J］. J Bacteriol, 2003, 185(19):57225734.

［12］HARDWICK S W, PANEFARRE J, DELUMEAU O, et al. Structural and functional characterization of partner switching regulating the environmental stress response in Bacillus subtilis［J］. J Biol Chem, 2007, 282(15): 11562-11572.

［13］SINGH V K, SCHMIDT J L, JAYASWAL R K, et al. Impact of sigB mutation on Staphylococcus aureus oxacillin and vancomycin resistance varies with parental background and method of assessment［J］. Int J Antimicrob Agents, 2003, 21(3):256-261.

［14］BANDOW J E, BROTZ H, HECKER M. Bacillus subtilis tolerance of moderate concentrations of rifampin involves the SigmaB-dependent general and multiple stress response［J］. J Bacteriol, 2002,184(2):459-467.

［15］MCGANN P, WIEDMANN M, BOOR K J. The alternative Sigma factor σ^B and the virulence gene regulator PrfA both regulator transcription of Listeria monocytogenes internalins［J］. Appl Environ Microbiol, 2007, 73(9): 2919-2930.

[1]	江玲丽，高有领，周向阳，白帆，方春，钱国英，方维焕. 单增李斯特菌膜裂解相关基因缺失突变株的构建及生物学特性鉴定[J]. 畜牧兽医学报, 2016, 47(4): 779-788.
[2]	江玲丽,陈健舜,李爱云,万婧,周向阳,钱国英,李彩燕,方维焕. 单核细胞增多性李斯特菌弱毒株的生物学特性鉴定[J]. 畜牧兽医学报, 2014, 45(2): 274-280.