雏鸭肝炎病毒感染后鸭CD8α基因及其通路相关基因表达变化

畜牧兽医学报

雏鸭肝炎病毒感染后鸭CD8α基因及其通路相关基因表达变化

徐琪¹，陈阳¹，黄正洋¹，童一宇¹，荣光辉¹，赵文明¹，张扬¹，李秀¹，段修军²，陈国宏^1*

（1. 江苏省动物遗传繁育与分子设计重点实验室, 扬州 225009； 2. 国家水禽种质资源基因库，泰州 225300）

收稿日期:2012-06-30 出版日期:2013-01-23 发布日期:2013-01-23
通讯作者: 陈国宏（1963-），男，江苏扬州人，博士，教授，主要从事动物遗传育种研究，E-mail: ghchen@yzu.edu.cn
作者简介:徐琪（1978-），男，江苏如东人，博士，主要从事家禽遗传育种研究，E-mail: xuqi@yzu.edu.cn
基金资助:
国家自然科学基金项目（31101704）；现代农业产业技术体系建设专项资金（CARS-43-3）；江苏高校优势学科建设工程资助项目（苏政办发【2011】137号）

Gene Expression Analysis of CD8α and Its Signaling Pathway Related Genes Treated with DHV in Duckling

XU Qi¹, CHEN Yang¹, HUANG Zheng-yang¹, TONG Yi-yu¹, RONG Guang-hui¹, ZHAO Wen-ming¹, ZHANG Yang¹, LI Xiu¹, DUAN Xiu-jun², CHEN Guo-hong^1*

(1. Jiangsu Key Laboratory of Animal Genetics ＆ Breeding and Molecular Design, Yangzhou 225009, China； 2. National Waterfowl Germplasm Resorurse, Taizhou 225300, China)

Received:2012-06-30 Online:2013-01-23 Published:2013-01-23

摘要/Abstract

摘要：

本研究旨在了解雏鸭肝炎病毒感染后鸭CD8α基因及其通路相关基因表达变化。通过对3日龄无母源抗体的金定鸭雏鸭感染雏鸭肝炎病毒（DHV），构建雏鸭肝炎感染实验动物模型，对雏鸭肝炎病毒发病组、未发病组和对照组的胸腺、肝脏、脾脏、肺、肾脏、大脑、小脑等组织CD8α基因及其信号通路上MHCⅠ、MHCⅡ、TNF-α等基因进行RT-qPCR检测。结果表明：在攻毒组（发病组和未发病组）扩增出DHV 3D基因的保守区域特异性条带，表明成功构建了雏鸭肝炎感染模型CD8α基因，RT-qPCR结果显示，CD8α、MHCⅠ和MHCⅡ基因在发病组各组织表现为不同程度的下调，在未发病组表现为不同程度的上调；而TNFα在发病组和非发病组的肝、脾、肺和肾等组织中表现为不同程度的上调。研究揭示了雏鸭肝炎病毒感染后CD8α基因及其通路相关基因表达变化，其结果有助于更好地了解duckCD8α基因在细胞免疫中表达调控作用。

Abstract:

This experiment was conducted to explore gene expression of CD8α and its signaling pathway related genes after virus infection. The experimental animal model of infected duck hepatitis virus (DHV) was constructed by using DHV to infect 3-day-old JinDing ducklings without maternal antibody. Then the gene expressions of CD8α, MHCⅠ, MHC Ⅱ and TNFα in some tissue of different groups of DHV were detected by RT-qPCR. The specific band of conservative regions in the DHV 3D gene was amplified in morbid group and non-morbid group. This outcome showed that the infected duck hepatitis virus animal model of CD8α gene was constructed successfully. The results of RT-qPCR demonstrated that the CD8α, MHCⅠ, MHC Ⅱ genes were up-regulated in non-morbid group and down-regulated in morbid group of all tissue in different degrees, while the TNF-α gene were up-regulated in morbid group of Liver, spleen, lung, and kidney. The research showed that the expression of the CD8α gene and related gene of its pathway was altered after duck hepatitis virus infection. The results contribute to a better understanding of the duck CD8α gene regulatory role in cellular immunity.

中图分类号:

S858.325.3

徐琪，陈阳，黄正洋，童一宇，荣光辉，赵文明，张扬，李秀，段修军，陈国宏. 雏鸭肝炎病毒感染后鸭CD8α基因及其通路相关基因表达变化[J]. 畜牧兽医学报, doi: .

XU Qi, CHEN Yang, HUANG Zheng-yang, TONG Yi-yu, RONG Guang-hui, ZHAO Wen-ming, ZHANG Yang, LI Xiu, DUAN Xiu-jun, CHEN Guo-hong. Gene Expression Analysis of CD8α and Its Signaling Pathway Related Genes Treated with DHV in Duckling[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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