全日制放牧BMY牛的活体采卵-体外胚胎生产-胚胎移植研究

摘要/Abstract

摘要：

为建立BMY牛的活体采卵(OPU)-体外胚胎生产(IVP)-胚胎移植(ET)快速扩繁技术体系，从人工草地全放牧牛群中选择健康的经产空怀BMY牛作为供体（n=14）集中OPU一次；卵母细胞经TCM-199体外成熟培养（IVM）、Fert-TALP液体外受精（IVF），受精卵用合成输卵管液（SOF）体外培养（IVC）生产胚胎；BMY牛受体（n=81）用CUE-MATE^TM孕酮阴道栓+EB+FSH+PG方法同期发情处理，对黄体合格的移植OPU-IVP来源鲜胚。研究结果表明：①OPU头均获卵母细胞23.50枚，获10枚以上卵母细胞的供体占85.7%；IVP的平均囊胚数和可用胚数分别为5.21和 4.00枚；OPU前8～9 d卵巢上有黄体（CL）的供体，头均获卵母细胞数及IVP的囊胚数、可移植胚数、囊胚率和可移植胚率分别比对照组高7.28枚（P＜0.05）、7.29枚（P＜0.05）、6.00枚（P＜0.05）、27.3%（P<0.01）和23.2%（P<0.01）；②受体黄体合格率69.1%（56/81），胚胎移植妊娠率37.5%（21/56），产犊率35.7%（20/56）；③受体在胚胎移植时注射GnRH，其妊娠率比对照组高16.2%（P＞0.05）。结果显示，BMY母牛具有良好的OPU潜力，但个体间差异较大；可通过严格供体选择及改善IVP技术等措施，实现OPU-IVP效率最大化。CUE-MATE^TM+EB+FSH+PG同期发情处理方法适于在牛规模化胚胎移植（ET）和人工授精（AI）中应用。综合应用OPU、IVP、同期发情和ET技术是加快优良BMY牛扩繁的重要途径之一。

Abstract:

The objective of this study was to establish a rapidly multiplication system of BMY cattle by the technology of ovum pick-up (OPU)-in vitro embryo production(IVP)-embryo transfer(ET). 14 BMY cattle with non-pregnancy, healthy and multiparous were selected for OPU once under whole year grazing in the artificial grasslands. Oocytes were matured in vitro in TCM-199 with 5% (v/v) fetal calf serum (FCS) and in vitro fertilization in Fert-TALP medium; zygotes were cultured in synthetic oviduct fluid (SOF) with 5% (v/v) FCS for embryo production. 81 BMY recipients were picked out for estrus synchronization treatment using CUE-MATE^TM (Controlled intravaginal progesterone-release device) + estradiol benzoate (EB) + follicle-stimulating hormone (FSH) + prostaglandin F₂_α analogue (PG), and recipients with a well-developed CL were transferred by BMY fresh embryos derived from OPU-IVP. The result showed that: ① Average donors with OPU were obtained 23.50 oocytes, and 85.7% donors offered more than 10 oocytes. Meanwhile, average blastocysts and available embryos of IVP were 5.21 and 4.00, respectively; Average oocytes, blastocysts, transferable embryos, rates of blastocyst and available embryo of donor ovaries with CL before OPU for 8 or 9 days were 23.57, 8.86, 7.00, 37.6% and 29.7%, respectively, significantly higher than those of control group (15.29, 1.57, 1.00, 10.3% and 6.5%, respectively, P<0.05 or 0.01). ② Ratios of corpus luteum (CL) up to A-B graded, pregnancy rate and calving rate were 69.1% (56/81), 37.5% and 35.7%, respectively. ③ Pregnancy rates of recipients with injection GnRH at the time of ET was 46.2%, higher than that of control group (without GnRH) (30.0%, P>0.05). The results indicated that BMY cows had better potential for OPU, but there was significant difference among individual donors. Moreover, the maximal efficiency of OPU-IVP was obtained by strictly selecting donors and improving technology of IVP and other measure. And the methods of CUE-MATE^TM + EB + FSH + PG for estrous synchronization were suited for applying to ET and AI in a large scale cattle herd. Finally, the technology of OPU, IVP, estrous synchronization and ET were synthetically applied to speed up the expanding propagation of fine BMY cattle.

中图分类号:

S823
S814.8

和占星，王喆，张继才，王安奎，金显栋，袁希平, Joaquim M. Garcia，Marina R. de Lima，何永珍，黄必志. 全日制放牧BMY牛的活体采卵-体外胚胎生产-胚胎移植研究[J]. 畜牧兽医学报, doi: .

HE Zhan-xing, WANG Zhe, ZHANG Ji-cai, WANG An-kui, JIN Xian-dong,YUAN Xi-ping, Joaquim M. Garcia, Marina R. de Lima, HE Yong-zhen, HUANG Bi-zhi. Ovum Pick up and in vitro Embryo Production and Embryo Transfer of BMY Beef Cattle under Whole Year Grazing System[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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