内蒙古绒山羊皮肤毛囊差异表达序列标签的筛选及分析

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (9): 1499-1503.doi:

内蒙古绒山羊皮肤毛囊差异表达序列标签的筛选及分析

苏立宁^1,2,3，李华^1,2，刘东军¹，旭日干^1*

（1. 内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室，呼和浩特 010021；
2. 佛山科学技术学院，佛山 528231； 3. 河北北方学院，张家口 075000）

收稿日期:2011-12-21 出版日期:2012-09-25 发布日期:2012-09-25
通讯作者: 旭日干，E-mail: xurg@imu.edu.cn
作者简介:苏立宁(1983-)，女，河北衡水人，助教，硕士，主要从事动物遗传育种研究,E-mail: sulining123@163.com;李华(1969-)，女，博士，教授，主要从事动物遗传育种研究，E-mail: okhua4@yahoo.cn。二者并列为第一作者
基金资助:
国家转基因项目(2008zx08008002)；博士后基金资助

Screening and Analysis of Differential Expressed Sequence Tags in Skin Hair
Follicles for Cashmere Goats

SU Li-ning^1,2,3, LI Hua^1,2, LIU Dong-jun¹, XU Ri-gan^1*

(1. The Key Laboratory for Mammalian Reproductive Biology and Biotechnology of Ministry
of Education, Inner Mongolia University, Hohhot 010021, China; 2. College of Life Science,
Foshan University, Foshan 528231, China; 3. Hebei North University，Zhangjiakou 075000, China)

Received:2011-12-21 Online:2012-09-25 Published:2012-09-25

摘要/Abstract

摘要： 旨在筛选和分析内蒙古绒山羊与毛和绒生长相关的差异基因，本研究采用mRNA差异显示技术（DDRT-PCR）研究内蒙古绒山羊胎儿期不同日龄皮肤中控制初级毛囊和次级毛囊不同分化的差异表达序列标签。结果，共筛选了15条差异表达序列标签（dbEST登录号JK711022-JK711036），其中3条ESTs与毛囊发育有关，5 条ESTs与次级毛囊和皮脂腺的发育有关。生物信息分析显示，JK711036与甲状腺激素受体相互作用蛋白12（TRIP12）基因有关，其他ESTs功能未知。结果表明，胎儿期初级毛囊和次级毛囊在多种代谢途径上都存在差异表达的基因，这将为进一步研究山羊毛和绒的形成分子机制奠定基础。

Abstract: To screen and analyze the genes associated with growth of goats’ wool and cashmere, differential display reverse transcription polymerase chain reaction (DDRT-PCR) technology was used to study the differentially expressed sequence tags ( ESTs ) at different embryo ages in skin primary and secondary follicles of Inner Mongolia Cashmere goats. 15 ESTs were identified (Acc_N: JK711022-JK711036) , of which 3 ESTs might play a role in the development of hair follicle, and 5 ESTs might be related to secondary follicles and sebaceous glands. The results of bioinformatics analysis showed that JK711036 shared homology with other species’ TRIP12 gene, but the function of other ESTs were unknown. All these were showed that many differential expressed genes was associated with primary and secondary wool follicles on metabolism pathways, which would establish molecular foundation for the formation of wool and cashmere in goats.

中图分类号:

S827
Q523.1

苏立宁, 李华, 刘东军 , 旭日干. 内蒙古绒山羊皮肤毛囊差异表达序列标签的筛选及分析[J]. 畜牧兽医学报, 2012, 43(9): 1499-1503.

SU Li-Ning, LI Hua, LIU Dong-Jun- , XU Ri-Gan. Screening and Analysis of Differential Expressed Sequence Tags in Skin Hair
Follicles for Cashmere Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1499-1503.

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参考文献

［1］苏立宁，李华，旭日干，等. 毛囊分子调控机制［J］. 生命的化学，2011, 31(3): 455-460.

［2］席海燕，周欢敏，郑琰. 绒山羊生长期次级毛囊 cDNA 消减文库的构建及其序列分析［J］. 中国农业科学，2009, 42(10): 3749-3754.

［3］尹俊，扈庭茂，李金泉，等. 内蒙古绒山羊毛囊兴盛期皮肤 cDNA 文库的构建及 KAP6-2 cDNA 的克隆［J］. 动物学研究，2004, 25(2): 166-171.

［4］李拥军，敖红，孙桂金，等. 羊体内发育与体外培养特定阶段胚胎基因差异表达的研究［J］. 畜牧兽医学报，2005, 36(12): 1281-1285.

［5］LATHE R, HARRIS A. Differential display detects host nucleic acid motifs altered in scrapieinfected brain ［J］. J Mol Biol, 2009, 392(3): 813-822.

［6］锡建中，赵振华，李少华，等. 山羊植入前胚胎发育相关基因的差异表达分析［J］. 江苏农业科学，2009, (2): 37-39.

［7］钱丽丽，贾青，李雪梅，等. 山羊高繁殖力相关基因的筛选与分析［J］. 畜牧兽医学报，2011, 42(4): 578-584.

［8］张燕军，尹俊，李金泉, 等. 内蒙古阿尔巴斯绒山羊毛囊结构及形态发生过程研究［J］. 中国农业科学, 2007, 40(5): 1017-1023.

［9］VON DER KAMMER H, ALBRECHT C, MAYHAUS M, et al. Identification of genes regulated by muscarinic acetylcholine receptors: application of an improved and statistically comprehensive mRNA differential display technique ［J］. Nucleic Acids Res, 1999, 27(10): 2211-2218.

［10］周汝真，李若瑜，余进. 白念珠菌抗氧化基因体外表达研究［J］. 中国真菌学杂志, 2009, 4(6): 325-329.

［11］王宏博，高雅琴. 绒山羊皮肤毛囊结构及其与产绒量关系的研究进展［J］. 安徽农业科学, 2008, 36(29): 12701-12703.

［12］PARK Y, YOON S K, YOON J B. The HECT domain of TRIP12 ubiquitinates substrates of the ubiquitin fusion degradation pathway ［J］. J Biol Chem, 2009, 284(3): 1540-1549.

［13］TANG W, MILLER M B, RICH S S, et al. Linkage analysis of a composite factor for the multiple metabolic syndrome ［J］. Diabetes, 2003, 52(11): 2840-2847.

［14］DOCO-FENZY M, LANDAIS E, ANDRIEUX J, et al. Deletion 2q36. 2q36. 3 with multiple renal cysts and severe mental retardation ［J］. Eur J Med Genet, 2008, 51(6): 598-607.

［15］RUBIO-ALIAGA I, PRZEMECK G K H, FUCHS H, et al. Dll1 haploinsufficiency in adult mice leads to a complex phenotype affecting metabolic and immunological processes ［J］. PloS One, 2009, 4(6): 506-512.