湖羊Lrh-1基因cDNA序列及组织表达谱分析

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (9): 1360-1368.doi:

湖羊Lrh-1基因cDNA序列及组织表达谱分析

王利红^1，2 ，高勤学²，张伟²，王锋^1*

（1.南京农业大学动物科技学院，南京 210095； 2.江苏畜牧兽医职业技术学院，泰州 225300）

收稿日期:2011-12-21 出版日期:2012-09-25 发布日期:2012-09-25
通讯作者: 王锋，E-mail：caeet@njau.edu.cn
作者简介:王利红（1975-），女，山西太原人，副教授，博士生，主要从事动物遗传繁育研究， E-mail：wanglihong345@yahoo.com.cn
基金资助:
国家肉羊产业技术体系（CARS-39）; 江苏省自然科学基金项目（BK2010356）

Lrh-1 cDNA Sequence Analysis and Tissue Expression in Hu Sheep

WANG Li-hong^1,2, GAO Qin-xue², ZHANG Wei², WANG Feng^1*

(1.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095,
China； 2. Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China)

Received:2011-12-21 Online:2012-09-25 Published:2012-09-25

摘要/Abstract

摘要： 本研究以湖羊肝脏为材料对肝受体类似物-1（Liver receptor homolog-1, Lrh-1;NR5A2）基因序列进行RT-PCR和RACE测定，并用DNAman、Tmpred、Signal P 3.0、Expasy等生物信息学分析软件和在线工具，对Lrh-1 cDNA序列及其蛋白的理化特性、跨膜结构、信号肽和二级结构进行生物信息学分析，同时采用实时荧光定量PCR方法检测湖羊Lrh-1基因的组织表达谱。结果表明，湖羊Lrh-1基因cDNA 序列全长1 488 bp，与牛的核酸序列相似度最高，为98%，编码区共编码495个氨基酸，氨基酸序列与牛、人、马、猴、犬、小鼠、褐鼠的氨基酸同源性分别为98%、97%、96%、97%、97%、86%和86%；Lrh-1 mRNA在湖羊脑、消化及生殖系统组织中均有表达且具有组织特异性，其中在下丘脑组织中的表达量相对最高。

Abstract: In the study, the full-length cDNA of the liver receptor homolog-1 (Lrh-1) was detected from liver of Hu sheep by reverse transcription PCR and RACE methods. Lrh-1 cDNA sequence, protein physicochemical properties, membrane structure, signal peptide and secondary structural characterisitics were analyzed and predicted with bioinformatics softwares and online tools ,such as DNAman, Tmpred, SignalP 3.0, TargetP 1.1, Expasy, and PSORT II prediction. The expression of Lrh-1 mRNA in different tissues of Hu sheep was also examined by quantitative real-time PCR. The results showed that the cDNA of Hu sheep Lrh-1 contained 1 488 nucleotides, and the nucleotide sequence had 98% homology with that of Bos taurus. It encoded 495 amino acid residues, which shared 98%, 97%, 96%, 97%, 97%, 86% and 86% identity with those of Bos taurus, Homo sapiens, Equus caballus, Macaca mulatta,Canis familiaris, Mus musculus and Rattus norvegicus, respectively. The relative expression level of Lrh-1 mRNA was detected in brain, digestive system and reproductive system, and the expression levels were showed differently and had tissue-specific. Relatively, Lrh-1 mRNA expression level was the highest in hypothalamus of Hu sheep.

中图分类号:

S826
S813.3

王利红,高勤学,张伟, 王锋. 湖羊Lrh-1基因cDNA序列及组织表达谱分析[J]. 畜牧兽医学报, 2012, 43(9): 1360-1368.

WANG Li-Hong-,GAO Qin-Xue-,ZHANG Wei, WANG Feng. Lrh-1 cDNA Sequence Analysis and Tissue Expression in Hu Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1360-1368.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2012/V43/I9/1360

参考文献

［1］FAYARD E, AUWERX J, SCHOONJANS K. LRH-1: an orphan nuclear receptor involved in development, metabolismand steroidogenesis ［J］. Trends Cell Biol, 2004, 14(5): 250-260.

［2］GALARNEAU L, PARE J F, ALLARD D, et al. The alphal-fetoprotein locus is activated by a nuclear receptor of the drosophila FTZ-F1 family ［J］.Mol Cell Biol, 1996,16(7):3853-3865.

［3］KUDO T, SUTON S. Molecular cloning of chicken FTZ-F1-ralated orphan receptors ［J］.Gene, 1997,197(1-2):261-268.

［4］BOERBOOM D, PILON N, BEHDJANI R, et al. Expression and regulation of transcripts encoding two members of the NRSA nuclear receptor subfamily of orphan nuclear receptors, steroidogenic factor-1 and NR5A2, in equine ovarian cells during the ovulatory process ［J］.Endocrinology,2000,141(12):4647-4656.

［5］LIU D, LE D Y, EKKER M, et al. Teleost FTZ-F1 homolog and its splicing variant determine the expression of the salmon gonadotropin II subunit gene ［J］. Mol Endocrinol, 1997, 11(7):877-890.

［6］ELLINGER Z H, HIHI A K, LAUDET V, et al. FTZ-F1-related orphan receptors in Xenopus laevis: transcriptional regulators differentially expressed during early embryogenesis ［J］.Mol Cell Biol,1994,14(4):2786-2797.

［7］NAK N T, TAKASE M, MIURAL I, et al. Two isoforma of FTZ-F1 messenger RNA: molecular cloning and their expression in the frog testis ［J］.Gene,2000,248(1-2):203-212.

［8］TANIGUCHI H, KOMIYAMA J, VIGER R S, et al. The expression of the nuclear receptors NR5A1 and NR5A2 and transcription factor GATA6 correlates with steroidogenic gene expression in the bovine corpus luteum ［J］.Mol Reprod Dev, 2009, 76(9): 873-880.

［9］顾月琴,张伟,王利红.小鼠Lrh-1基因CDS区序列克隆及分析［J］.安徽农业大学学报, 2011,(3):372-375.

［10］温海霞，刘国艺，倪江.孤儿核受体同系物-1以及与雌激素相互调节作用［J］.生殖医学杂志,2007,16(2):124-128.

［11］张英杰.羊生产学［M］. 北京：中国农业大学出版社, 2010.

［12］BECKER A M, ANDRE E, DELAMARTER J F. Identification of nuclear receptor mRNAs by RTPCR amplification of conserved zinc-finger motif sequences ［J］. Biochem Biophys Res Comm, 1993, 194:1371-1379.

［13］WANG Z N, BASSETT M, RAINEY W E. Liver receptor homologue-1 is expressed in the adrenal and can regulate transcription ［J］. J Mol Endocrinol, 2001, 27(2):255-258.

［14］GRGUREVIC N, TOBET S, MAJDIC G. Widespread expression of liver receptor homolog 1 in mouse brain ［J］. Neuro Endocrinol Lett, 2005, 26:541-547.

［15］HIGASHIYAMA H, KINOSHITA M, ASANO S. Expression profiling of liver receptor homologue 1(LRH-1) in mouse tissues using tissue microarray ［J］. J Mol Hist, 2007, 38:45-52.

［16］VAN DEN TOP M, SPANSWICK D. Integration of metabolic stimuli in the hypothalamic arcuate nucleus［J］. Prog Brain Res, 2006, 153:141-154.

［17］韩正康.家畜生理学［M］.北京：中国农业出版社，1984.

［18］LUO Y, LIANG C P, TALL A R. The orphan nuclear receptor LRH-1 potentiates the sterol-mediated induction of the human CETP gene by liver X receptor ［J］. J Biol Chem, 2001, 276: 24767-24773.

［19］SCHOONJANS K, ANNICOTTE JS, HUBY T, et al. Liver receptor homolog 1 controls the expression of the scavenger receptor class B type I ［J］. EMBO Rep,2002, 3:1181-1187.

［20］ANNICOTTE J S, FAYARD E, SWIFT G H, et al. Pancreatic-duodenal homeobox 1 regulates expression of liver receptor homolog 1 during pancreas development ［J］. Mol Cell Biol, 2003,23:6713-6724.

［21］FAYARD E, SCHOONJANS K, ANNICOTTE J S, et al. Liver receptor homolog 1 controls the expression of carboxy 1 ester lipase ［J］.J Biol Chem, 2003, 278: 35725-35731.

［22］CAO G, GARCIA C K, WYNE K L, et al. Structure and localization of the human gene encoding SR-BI/CLA-1. Evidence for transcriptional control by steroidogenic factor 1［J］.J Biol Chem, 1997, 272:33068-33076.

［23］HARFOOT C G. Anatomy physiology and microbiology of the ruminant tract in “Lipid metabolism in ruminant animals”［M］. W W Christie, Perganian Press, 1981:1-19.

［24］BOERBOOM D, PILON N, BEHDJANI R, et al. Expression and regulation of transcripts encoding two members of the NR5A nuclear receptor subfamily of orphan nuclear receptors, steroidogenic factor-1 and NR5A2, in equine ovarian cells during the ovulatory process ［J］. Endocrinology, 2000,141:4647-4656.

［25］DUGGAVATHI R, VOLLE D H, MATAKI C, et al. Liver receptor homolog 1 is essential for ovulation ［J］. Genes Dev, 2008, 22(14):1871-1876.

［26］PENG N, KIM J W, RAINEY W E, et al. The role of the orphan nuclear receptor, liver receptor homologue-1,in the regulation of human corpus luteum3b-hydroxysteroid dehydrogenase type II ［J］. J Clin Endocrinal Metab, 2003, 88: 6020-6028.

[1]	张园，高峰,刘迎春，傲丽格日玛. 妊娠后期胎儿宫内生长受限对蒙古绵羊胎儿胸腺生长发育的影响[J]. 畜牧兽医学报, 2012, 43(11): 1710-1715.
[2]	林婄婄, 高中元, 袁亚男, 武建亮, 刘宝凤, 周沙沙, 乔利英, 刘建华, 梁琛, 刘文忠. PPARα和PPARγ基因在不同脂尾型绵羊脂肪组织中的发育性表达研究[J]. 畜牧兽医学报, 2012, 43(9): 1369-1376.
[3]	刘燕, 罗奋华, 包佳婧, 刘林洪, 单飞彪, 吴应积. 绵羊Gfrα1基因的部分cDNA克隆与抗原表位多肽的原核表达[J]. 畜牧兽医学报, 2012, 43(9): 1377-1384.