O型口蹄疫抗体金标检测试纸条判定标准的确立

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (9): 1415-1421.doi:

O型口蹄疫抗体金标检测试纸条判定标准的确立

智晓莹^1,2，任维维^1,3，祁光宇^1,2，吕建亮¹，梁仲¹，蒋韬^1*

（1. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室，
兰州 730046; 2. 中农威特生物科技股份有限公司，兰州 730046; 3. 兰州大学第一临床医学院，兰州730000）

收稿日期:2012-01-04 出版日期:2012-09-25 发布日期:2012-09-25
通讯作者: 蒋韬，副研究员，E-mail：pcrjiang@163.com
作者简介:智晓莹(1981-)，女，山东龙口人，助理研究员，硕士，主要从事动物病毒学与免疫学的研究，E-mail: zhixiaoying333@163.com
基金资助:
甘肃省科技厅资助；甘肃科技重大技术专项(1002NKDA037)

Establishment of Judging Standard of Colloidal Gold-based Immunochromatographic
Assay Strip for Detection Antibodes of Serotype O Foot-and-mouth Disease

ZHI Xiao-ying^1,2, REN Wei-wei^1,2, QI Guang-yu^1,2, LV Jian-liang¹, LIANG Zhong¹, JIANG Tao^1*

(1. Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of
Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of
Agricultural Sciences, Lanzhou 730046, China; 2. China Agricultural Vet.Bio.Science and
Technology Co.,Ltd., Lanzhou 730046, China; 3. The First Clinical Medicine College of
Lanzhou University, Lanzhou 730000, China)

Received:2012-01-04 Online:2012-09-25 Published:2012-09-25

摘要/Abstract

摘要： 本试验旨在迅速、准确检测口蹄疫阴性血清和阳性血清，确立O型口蹄疫抗体金标检测试纸条的判定标准。通过3批次O型口蹄疫抗体金标检测试纸条，对180份猪、牛、羊口蹄疫阴性血清，197份猪、牛O型口蹄疫弱阳性血清，317份猪、牛、羊O型口蹄疫阳性血清，223份牛Asia 1、A型口蹄疫阳性血清，600份田间血清样品进行检测，同时用O型口蹄疫液相阻断ELISA和O型口蹄疫正向间接血凝2种方法检测相同的血清样品。对3种方法检测的结果进行分析，并且将试纸条的检测线和质控线的显色情况与O型口蹄疫液相阻断ELISA的抗体滴度相比较。确立了试纸条的诊断标准：试纸条抗体滴度1∶2-为口蹄疫抗体阴性；试纸条抗体滴度1∶8+为O型口蹄疫阳性血清。根据试纸条的显色结果与液相阻断ELISA的结果的关系，绘制了比色卡。试纸条的判定标准检测血清结果与液相阻断ELISA和正向间接血凝结果分别进行统计学分析,试纸条与液相阻断ELISA和正向间接血凝相关性分别为y = 1.142x + 0.742 1，y = 1.184 5x + 0.862 3；相关系数（r）分别为0.922 1和0.903 3。结果显示，O型口蹄疫抗体金标检测试纸条的判定标准的确立和比色卡的绘制，实现半定量分析，更加迅速、准确，适于实验室、基层兽医站和养殖场使用。

Abstract: The objective of the study was to establish a judging standard of colloidal gold-based immunochromatographic assay strip (GICA) for detecting antibodies of serotype O of foot-and-mouth disease (FMD) rapidly. The GICA of 3 batches were tested. There were a hundred and eighty FMD negative serum samples (containing pig, cattle and sheep), a hundred and ninety-seven FMD weak positive serum samples (containing pig, cattle), three hundred and seventeen FMD serotype O positive serum samples (containing pig, cattle and sheep), two hundred and twenty-three FMD serotype A and Asia 1 positive cattle sera, six hundred filed samples. To compare three methods of GICA, liquid-phase competition ELISA (LB-ELISA) of FMDV serotype O and Indirect Hemagglutination Test (IHA) of FMDV serotype O for detecting antibodies of FMD serotype O, all the serum samples were detected, the judging standard was established. According to the color of test line and control line, compared with the titers of LB-ELISA, the colorimetric card was drawn. The judging standard of GICA was as follows: Using a 1:2 dilution of the sample, the result was negative, and the anti-FMDV serum was negative; Using a 1:8 dilution of the sample, the result was positive, which implied that the serum had serotype O FMDV positive antibodies. Compared with GICA and LB-ELISA, the colorimetric card was drawn which was helpful for reading results. The correlation of the GICA and LPBE was y = 1.142x + 0.742 1; the correlation coefficient (r) was 0.922 1; the correlation of the GICA and IHA was y = 1.184 5x + 0.862 3; the correlation coefficient (r) was 0.903 3. These results indicated that the judging standard of GICA was prior to detecting antibodies of FMD serotype O; the colorimetric card was easy to read, which was just monitoring the anti-FMD serotype O antibodies of immunized animals fast and accurately. This judging standard could promote the qualitative analysis to semi-quantitative analysis, and it had provided that it was more suitable for using in laboratory and the field.

中图分类号:

S854.43

智晓莹, 任维维, 祁光宇, 吕建亮, 梁仲, 蒋韬. O型口蹄疫抗体金标检测试纸条判定标准的确立[J]. 畜牧兽医学报, 2012, 43(9): 1415-1421.

ZHI Xiao-Ying, REN Wei-Wei, QI Guang-Yu, 吕Jian-Liang,LIANG Zhong, JIANG Tao. Establishment of Judging Standard of Colloidal Gold-based Immunochromatographic
Assay Strip for Detection Antibodes of Serotype O Foot-and-mouth Disease[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1415-1421.

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