鹅DHRS7基因克隆、序列分析及其在鹅肥肝形成过程中的表达特点

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (9): 1385-1391.doi:

鹅DHRS7基因克隆、序列分析及其在鹅肥肝形成过程中的表达特点

何桦，潘志雄，刘贺贺，李乐，夏露，胡深强，鲁凯，董霞，李亮，王继文^*

（四川农业大学动物遗传育种研究所，成都 611130）

收稿日期:2011-12-26 出版日期:2012-09-25 发布日期:2012-09-25
通讯作者: 王继文，E-mail：wjw2886166@163.com
作者简介:何桦（1982-），男，重庆人，助理研究员，硕士，主要从事水禽遗传育种研究，E-mail: hehua023@126.com
基金资助:
国家水禽产业技术体系（CARS-43-6）

Molecular Cloning and Bioinformatics Analysis of Goose DHRS7 and Its Expression
Characteristics during Goose Fatty Liver Formation

HE Hua, PAN Zhi-Xiong, LIU He-He, LI Le, XIA Lu, HU Shen-Qiang, LU Kai, DONG Xia, LI Liang, WANG Ji-Wen^*

(Institute of Animal Breeding & Genetic, Sichuan Agricultural University,
Chengdu 611130, China)

Received:2011-12-26 Online:2012-09-25 Published:2012-09-25

摘要/Abstract

摘要： 为获得鹅DHRS7基因全长cDNA，并探讨该基因是否参与鹅肥肝的形成过程。本研究应用抑制消减杂交文库获得鹅DHRS7基因部分EST序列，采用RACE技术扩增了鹅该基因全长cDNA，并对其序列进行了生物信息分析；用荧光定量PCR技术检测了DHRS7在鹅肝脏、皮脂、腹脂等10个组织中的差异表达，以及填饲对鹅肝脏中DHRS7表达变化的影响。结果发现，鹅DHRS7 基因cDNA全长1 279 bp，含一个完整的开放阅读框1 011 bp（可编码336个氨基酸），25 bp的5′UTR和243 bp的 3′UTR序列；生物信息学分析结果显示，鹅DHRS7含有跨膜结构域以及NADP结合位点，且在这些功能区域变异较少；荧光定量结果显示，DHRS7在鹅肝脏和脂肪组织中都有较高表达，填饲后鹅肝脏中DHRS7的表达水平显著上调（P<0.05）。以上结果表明，DHRS7在鹅肥肝形成过程中具有重要的作用。

Abstract: In order to elucidate the roles of DHRS7 gene in formation of goose fatty liver, the goose DHRS7 gene full-length cDNA sequence was cloned by RACE method, and then was analyzed by bioinformatics method. Meanwhile, expression of DHRS7 in goose different tissues and organs was analyzed using qRT-PCR method, as well as the mRNA expression influenced by forcing-feed was analyzed in goose liver. Results showed that the full sequence of goose DHRS7 gene was 1 297 bp, including an coding domain sequence (CDS) encoding for 336 AA, and a 25 bp 5′ UTR and a 243 bp 3′UTR. Results of bioinformatics analysis showed that the geese DHRS7 posses a transmembrane region and a NADP binding region, on which functional region there is few variants. The DHRS7 had a higher expression level in geese liver and adipose tissues. And the expression of DHRS7 up-regulated significantly in geese liver tissues after a force feeding (P<0.05). The results indicate that the goose DHRS7 gene may play an important role during formation of geese fatty liver.

中图分类号:

S835
S813.3

何桦, 潘志雄, 刘贺贺, 李乐, 夏露, 胡深强, 鲁凯, 董霞, 李亮, 王继文. 鹅DHRS7基因克隆、序列分析及其在鹅肥肝形成过程中的表达特点[J]. 畜牧兽医学报, 2012, 43(9): 1385-1391.

HE Hua, PAN Zhi-Xiong, LIU He-He, LI Le, XIA Lu, HU Shen-Qiang, LU Kai, DONG Xia, LI Liang, WANG Ji-Wen. Molecular Cloning and Bioinformatics Analysis of Goose DHRS7 and Its Expression
Characteristics during Goose Fatty Liver Formation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1385-1391.

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