畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (5): 838-842.doi:

• 研究简报 • 上一篇    

1株山羊内源性肺腺瘤反转录病毒的序列测定与分析

周艳喜1，李靖1，敖威华2，刘霄卉1，于立新1，幺宏强1，马学恩1*   

  1. 1.内蒙古农业大学兽医学院，呼和浩特，010018； 2.内蒙古出入境检验检疫局，呼和浩特，010020
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-05-28 发布日期:2012-05-28
  • 通讯作者: 马学恩

Cloning and Sequence Analysis of Goats Endogenous Pulmonary Adenomatosis Retrovirus

ZHOU Yanxi1 ，LI Jing1，AO Weihua2 ，LIU Xiaohui1，YU Lixin1, YAO Hongqiang1，MA Xueen1*   

  1. 1. College of Veterinary Medicine，Inner Mongolia Agricultural University, Huhhot 010018, China；2. Inner Mongolia EntyExit Inspection and Quarantine Bureau, Huhhot 010020, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28
  • Contact: MA Xueen

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摘要： 参照内源性绵羊肺腺瘤病毒株enJSRV20（EF680302.1）全基因组序列设计合成3对引物，以山羊基因组DNA为模板，应用PCR技术扩增山羊内源性病毒基因片段，分别连接PMD18T载体并测序，完成了1株山羊内源性肺腺瘤反转录病毒全基因序列的测定。结果分析显示测定序列全长7 480 bp，获得的序列与内源性绵羊肺腺瘤病毒株enJSRV18（EF680301.1）的核苷酸相似性为93.4% ，与外源性绵羊肺腺瘤病毒株JS7（AF357971.1）的核苷酸相似性为88.1%。根据pro基因序列构建系统进化树，结果显示扩增序列与内源性绵羊反转录病毒株enJSRV5（EF680305.1）的亲缘关系最近。本研究有助于内源性肺腺瘤反转录病毒感染性克隆的构建和内源性肺腺瘤反转录病毒功能的研究。

Abstract: Three pairs of primers were designed according to the sequence of strain enJSRV20. Sequence of goats enJSRV was amplified by PCR technology and these PCR products were cloned into PMD18T vector and then sequenced . The complete genome of goats enJSRV was obtained successfully. Analysis results showed that the sequence of goats enJSRV was 93.4% similarity with enJSRV18 and 88.1% similarity with strain JS7, respectively. Phylogenetic analysis according to pro gene showed that goats enJSRV had higher homology with strain enJSRV5 .It will be useful for the study of constructing infectious clone and elucidation the function of endogenous pulmonary adenomatosis retrovirus.

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CN 11-1985/S
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