乳酸锌对猪空肠上皮细胞IPECJ2增殖及相关调控基因mRNA表达的影响

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (5): 740-747.doi:

乳酸锌对猪空肠上皮细胞IPECJ2增殖及相关调控基因mRNA表达的影响

韩国全1，余冰1,2，陈代文1,2*，相振田1，亓宏伟1，陈洪1，毛倩1，毛湘冰1，黄志清1

1. 四川农业大学动物营养研究所，雅安 625014； 2. 四川农业大学动物抗病营养教育部重点实验室，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 陈代文

Effect of Zinc Lactate on Cell Proliferation and Relatedregulatory Genes mRNA Expression in Porcine Jejunal Epithelial Cells IPECJ2

HAN Guoquan1, YU Bing1，2, CHEN Daiwen1，2*, XIANG Zhentian1, QI Hongwei1, CHEN Hong1, MAO Qian1, MAO Xiangbing1, HUANG Zhiqing1

1. Institue of Animal Nutrition, Sichuan Agricultural University, Ya’an 625014, China; 2. Key Laboratory of Animal Diseaseresistant Nutrition of Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28

摘要/Abstract

摘要： 旨在探讨乳酸锌对猪空肠上皮细胞增殖及相关调控基因ZnT2、DMT1、IREG1、MT1和ZIP4 mRNA表达的影响。用乳酸锌的锌浓度分别为50、100、150、200 mg·L-1的培养基培养IPECJ2细胞，采用比色法测定分析细胞增殖变化；用实时荧光定量RTPCR方法检测ZnT2、DMT1、IREG1、MT1及ZIP4 mRNA表达，以TBP mRNA的表达水平作为内参对照。在细胞培养前36 h，乳酸锌对IPECJ2细胞基本没有影响，随锌浓度递增细胞增殖幅度升高；乳酸锌处理IPECJ2细胞后， ZnT2、DMT1、IREG1及MT1 mRNA表达随锌浓度增高而升高，ZIP4 mRNA表达则随锌浓度增高而降低。添加乳酸锌可以促进IPECJ2细胞增殖，上调ZnT2、DMT1、IREG1、MT1 mRNA表达，下调ZIP4 mRNA表达。

Abstract: The present study was conducted to evaluate the effects of zinc lactate on cell proliferation of jejunal epithelial cells IPECJ2 and mRNA expression of relatedregulatory genes in porcine. IPECJ2 cells were cultured with mediums added with zinc lactate by the zinc concentrations of 50, 100, 150 and 200 mg·L-1, respectively. Cell proliferation was estimated by Colorimetric analysis. Simultaneously, realtime quantitative RTPCR was applied to detect the mRNA expression of relatedregulatory genes (ZnT2, DMT1, IREG1, MT1 and ZIP4), and TBP mRNA level was used as the control. During the first 36 hours after treatment with zinc lactate, there was almost no effect on IPECJ2 cell proliferation. Subsequently, with increasing of the concentration of zinc, the rate of cell proliferation gradually increased. At the same time, after IPECJ2 cell was treated with zinc lactate, the expression of ZnT2, DMT1, IREG1and MT1 mRNA was increased with the increase of the concentration of zinc while the expression of ZIP4 mRNA decreased. Zinc lactate could promote cell proliferation of IPECJ2, upregulated the mRNA expression of ZnT2, DMT1, IREG1, MT1 and downregulated the mRNA expression of ZIP4.

韩国全;余冰;陈代文;相振田;亓宏伟;陈洪;毛倩;毛湘冰;黄志清. 乳酸锌对猪空肠上皮细胞IPECJ2增殖及相关调控基因mRNA表达的影响[J]. 畜牧兽医学报, 2012, 43(5): 740-747.

HAN Guoquan;YU Bing;CHEN Daiwen;XIANG Zhentian;QI Hongwei;CHEN Hong;MAO Qian;MAO Xiangbing;HUANG Zhiqing. Effect of Zinc Lactate on Cell Proliferation and Relatedregulatory Genes mRNA Expression in Porcine Jejunal Epithelial Cells IPECJ2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(5): 740-747.

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