Abstract: Apicomplexa is a protozoan class of obligate intracellular parasites, including Toxoplasma gondii, Cryptosporidium spp., Plasmodium spp., Babesia spp. and Eimeria spp., many of them are important pathogens to human and animal. They have conserved subcellular structures and invasion mechanism. The results of lots of researches demonstrate that the invasion mechanisms are mediated by many invasionassociated factors, including the proteins secreted from micronemes, rhoptries and dense granules. The researches about these proteins make great progress with the development of bioinformatics and biotechnologies. In this paper, we review the latest progress of invasionassociated factor combining with our research results in recent years.