IGFI与IGFBP1基因对京海黄鸡生长性状的遗传效应分析

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (1): 152-158.doi:

IGFI与IGFBP1基因对京海黄鸡生长性状的遗传效应分析

赵秀华1,2，王金玉1,2*，张跟喜1,2，魏岳1,2，顾玉萍3，俞亚波3，施会强3

1.扬州大学动物科学与技术学院，扬州225009； 2.江苏省动物遗传繁育与分子设计重点实验室，扬州 225009； 3.江苏京海禽业集团有限公司，海门 226103

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-15 发布日期:2012-01-15
通讯作者: 王金玉

Genetic Effects of IGFI and IGFBP1 Genes on the Growth Traits in Jinghai Yellow Chicken

ZHAO Xiuhua1,2, WANG Jinyu1,2*, ZHANG Genxi1,2,
WEI Yue1,2, GU Yuping3 , YU Yabo3,SHI Huiqiang3

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; 3. Jiangsu Jinghai Poultry Group Co., Ltd., Haimen 226103,China

Received:1900-01-01 Revised:1900-01-01 Online:2012-01-15 Published:2012-01-15
Contact: WANG Jinyu

摘要/Abstract

摘要： 旨在对IGFI和IGFBP1基因部分SNPs与鸡生长性状进行关联分析。试验以京海黄鸡为材料，采用PCRSSCP方法检测2个候选基因的单核苷酸多态性（SNPs），采用一般线性模型（GLM）分析基因型与生长性状的遗传效应。结果表明，IGFI基因外显子3序列的60 bp处有A→G的点突变，在京海黄鸡中检测到AA、AB、BB 3种基因型，A等位基因频率为0.613，B等位基因的频率为0.387；IGFBP1基因外显子2序列的21 bp处有A→T的点突变，104 bp处有T→C的突变，在京海黄鸡中检测到CC、CD和DD 3种基因型，C等位基因频率为0.558，D等位基因频率为0.442。IGFI基因BB基因型个体4周龄体质量显著高于AA和AB型个体（P<0.05）；IGFBP1基因DD基因型个体8周龄体质量显著高于CC基因型个体（P<0.05），4、12和16周龄体质量差异极显著（P<0.01）。因此，推测这些SNPs对京海黄鸡生长性状具有一定的影响，应用于鸡育种过程中的标记辅助选择可以加快育种进程。

Abstract: The aim of this study was to analyze the association of SNPs in IGFI and IGFBP1 genes with chicken growth traits.PCRSSCP approach was applied to assess the single nucleotide polymorphisms(SNPs) and the general linear model was used to analyze genetic effects between different genotypes and growth traits of the Jinghai Yellow chicken. For the IGFI gene, three genotypes (AA, AB and BB) were detected in Jinghai Yellow chicken population, the frequency of A and B alleles was 0.613 and 0.387, respectively. Sequencing revealed one mutation (G60A) of IGFI gene in the genotype BB in comparison to the genotype AA. For IGFBP1 gene, three genotypes (CC, CD and DD) were detected in Jinghai Yellow chicken population, the frequency of C and D alleles was 0.558 and 0.442, respectively. Sequencing revealed two mutations (A21T, T104C) of the IGFBP1 gene in the genotype DD in comparison to the genotype CC. The results showed that individuals with BB genotype of the IGFI gene had higher bodyweight at 4 weeks compared to individuals with AA and AB genotypes (P<0.05); The individuals with DD genotype of the IGFBP1 gene had significantly higher bodyweight at 4, 12, 16 weeks compared to the individuals with CC genotype (P<0.01). The results suggest that SNPs of IGFI and IGFBP1 genes have certain effects on growth traits of Jinghai Yellow chicken, which can be used in markerassisted selection to accelerate the chicken breeding progress.

赵秀华;王金玉;张跟喜;魏岳;顾玉萍;俞亚波;施会强. IGFI与IGFBP1基因对京海黄鸡生长性状的遗传效应分析[J]. 畜牧兽医学报, 2012, 43(1): 152-158.

ZHAO Xiuhua;WANG Jinyu;ZHANG Genxi;WEI Yue;GU Yuping;YU Yabo;SHI Huiqiang. Genetic Effects of IGFI and IGFBP1 Genes on the Growth Traits in Jinghai Yellow Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(1): 152-158.

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