Abstract: The contents of GSHPx in the whole blood of sika deer within different physiological period and the technique for purification GSHPx, were studied for developing antiaging resources of deer blood and laid a theoretical basis. The contents of GSHPx in the blood cells, plasma, dissolved blood and cell membrane were detected by using DTNB method and the contents of GSHPx in the whole blood were calculated. A 10%100% of saturation (NH4)2SO4 saltingout and column chromatography technology were used to purify the GSHPx and the SDSPAGE was used to measure its subunit molecular weight. The results showed that the highest contents of GSHPx appeared during the time of antler developing period ((1 973.07±25.43) U·mL-1), which was significantly higher (P<0.01) than that in the service period ((1 727.74±12.46) U·mL-1). However, no significant difference (P>0.05) was found between the value in antler developing period and the preantler period ((1 961.83±16.54) U·mL-1). GSHPx initial specific activity was 24.9 U·mg-1 and 0.37 mg·mL-1 purified GSHPx was achieved. Finally specific activity was 1 347.7 U·mg-1 and 54.1 times purification rate and 25% recovery rate were achieved. The relative molecular weight of subunit for GSHPx was 17.2 kDa. The results demonstrated that there are some certainly correlation between the change of GSHPx content and the physiological period of the deer. The saturation of 40%80% (NH4)2SO4 is the best condition for GSHPx purification.