Abstract: The study was conducted to analyze mutations and search protein motifs of β1 domain of SLADQB gene in Rongchang pigs. The mutation analysis of the sequences of β1 domain of SLADQB gene from 53 Rongchang pigs was performed by multiple sequence alignment. The protein motifs of β1 domain of SLADQB gene was predicted by on line softwares. The results showed that 9 single nucleotide insertion sites and 16 single nucleotide deletion sites and 89 single nucleotide polymorphism sites were observed in 222 base pairs. 37 missense mutations were observed in 74 amino acid residues resulted by 37 SNPs. The change of amino acid type happened in 24 of 37 missense mutations. There were 174 protein motifs including 7 types in 50 amino acid sequences of β1 domain of SLADQB gene. The maximum number of protein motifs for a single sequence was 12 and the minimum number was 2. The mutations of protein motifs were observed at the 9th, 26th，45th，53th and 61th amino acid residues, which involing in 5 types of protein motifs. These results indicated that β1 domain of SLADQB gene in Rongchang pigs owned abundant genetic diversity and diversified protein motifs.