Abstract: The liposomeencapsulated mChIL18 was firstly applied on its immunoenhancement for Newcastle disease vaccine with different concentrations. In the experiment, onedayold SPF chickens were randomly divided into six groups, the blank control group, the NDV vaccine group, the NDV vaccine plus 0.2 mL mChIL18 group, the NDV vaccine plus 0.1 mL mChIL18 group, the NDV vaccine plus 0.3 mL mChIL18 group and the NDV vaccine plus vector control group．All groups were immunized on the seven days old．After that, peripheral blood was collected with anticoagulation, and the antibody level, CD4+ and CD8+ T lympholeukocytes were detected by HI and FACS at each week, respectively. Standard Virulent Strain F48E9 (100 times ELD50 per chicken) was given to the chickens at last vaccination. The results indicated that the best concentration of mChIL18 liposomes is 0.2 mL per chicken． The mChIL18 liposomes can not only enhance the cellular immune response significantly, but also enhance the humoral response, improving the protective rate of ND vaccine.