济宁青山羊生后发育阶段GnRH与GnRHR阳性细胞在下丘脑分布规律的研究

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (8): 1186-1191.doi:

• 遗传繁育 • 上一篇下一篇

济宁青山羊生后发育阶段GnRH与GnRHR阳性细胞在下丘脑分布规律的研究

刘宵^1,2，王树迎^1,2*，尹玉涛^1,2，张德敏^1,2，黄丽波^1,2，侯衍猛^1,2

1. 山东农业大学动物医学院，泰安 271000； 2. 山东农业大学动物科技学院动物生物技术与疾病防治重点实验室，泰安 271000

收稿日期:2011-12-11 修回日期:1900-01-01 出版日期:2012-08-24 发布日期:2012-08-24
通讯作者: 王树迎

Distribution Patterns of GnRH and GnRHR Positive Cells in Hypothalamus at Different Postnatal Stages in Jining Gray Goats

LIU Xiao ^1,2, WANG Shuying ^1,2*, YIN Yutao ^1,2, ZHANG Demin^1,2,

HUANG Libo^1,2, HOU Yanmeng ^1,2

1. College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271000, China; 2. Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271000, China

Received:2011-12-11 Revised:1900-01-01 Online:2012-08-24 Published:2012-08-24
Contact: WANG Shuying

摘要/Abstract

摘要： 为研究济宁青山羊生后发育阶段GnRH及GnRHR在下丘脑内的形态学分布和变化规律，采用链霉亲和素生物素过氧化物酶复合物（Strept Avidin BiotinPeroxidase Complex, SABC）免疫组织化学方法，对0、2、4和6月龄雌性济宁青山羊下丘脑中GnRH及GnRHR的分布进行了同步研究。结果显示，GnRH和GnRHR免疫阳性细胞在下丘脑内广泛存在，主要分布于视前内侧区、乳头体、视上核和视交叉上核。随月龄增长，GnRH和GnRHR阳性细胞不断增大，数量不断增多，其中0～2月龄是最快的时期。结果提示，下丘脑分泌的GnRH及GnRHR对生后发育阶段青山羊性成熟的启动及维持有重要作用。

Abstract: In order to detect the morphological changes and distribution patterns of GnRH and GnRHR in hypothalamus at different postnatal stages in Jining Gray goats, immunohistochemical Strept Avidin BiotinPeroxidase Complex (SABC) threestep method was used to study the distribution of GnRH and GnRHR in hypothalamus in goats at 0, 2, 4 and 6 months old. The results showed that GnRH and GnRHR positive cells were widely scattered in hypothalamus. Most of the positive cells were distributed in medial preoptic area (MPA), mamillary nucleus (MB), supraoptic nucleus (SON) and suprachiasmatic nucleus (SCN). The size and number of positive cells were increased with the increase of month old. The numbers of positive GnRH and GnRHR cells were significantly higher at 2 month old (puberty) than that at birth. The results suggest that GnRH and GnRHR secreted by hypothalamus might play a pivotal role in the onset of puberty and the maintenance of maturation in Jining Gray goat at the postnatal developing stage.

刘宵;王树迎;尹玉涛;张德敏;黄丽波;侯衍猛;. 济宁青山羊生后发育阶段GnRH与GnRHR阳性细胞在下丘脑分布规律的研究[J]. 畜牧兽医学报, 2012, 43(8): 1186-1191.

LIU Xiao;WANG Shuying;YIN Yutao;ZHANG Demin; HUANG Libo;HOU Yanmeng;. Distribution Patterns of GnRH and GnRHR Positive Cells in Hypothalamus at Different Postnatal Stages in Jining Gray Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(8): 1186-1191.

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