畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (1): 71-81.doi:

• 动物营养 • 上一篇    下一篇

茶渣单宁含量对绵羊养分消化利用与氮代谢参数的影响

潘发明1,李发弟1,2*,郝正里1,郑琛1,董淑慧1   

  1. 1.甘肃农业大学动物科学技术学院,兰州 730070;2.甘肃省饲料工程技术研究中心,兰州 730000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 李发弟

Effects of Tannin Content in Residue of Tealeaves on Digestion and Utilization of Nutrients and Metabolic Parameters of Nitrogen in Sheep

PAN Faming1, LI Fadi1,2*, HAO Zhengli1,ZHENG Chen1, DONG Shuhui1   

  1. 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2. Gansu Feed Engineer Technology Research Center, Lanzhou 730000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-01-15 Published:2012-01-15
  • Contact: LI Fadi

摘要: 旨在研究茶渣单宁含量对绵羊养分消化利用与氮代谢参数的影响。本研究分析了茶渣(RTL)的营养成分和单宁含量;用体外法测定6种配比茶渣/大豆粕混合料(0∶10、1∶9、2∶8、3∶7、4∶6、10∶0,其中茶渣缩合单宁(RTLCT)含量依次为0.00、0.16、0.31、0.47、0.63和1.57 g·kg -1DM)的瘤胃液与瘤胃液+HCl胃蛋白酶干物质(DM)与粗蛋白质(CP)降解率(rdDM、tdDM、rdCP、tdCP);用4 只安装永久性瘤胃瘘管的甘肃高山细毛羊羯羊(1 岁半,体质量(29±2.55)kg),按4×4 拉丁方方案进行试验,研究了饲粮RTLCT水平(A、B、C、D分别为0.00、0.77、1.53、2.30 g·kg-1DM)对绵羊养分消化利用与氮代谢参数的影响,共4个15 d的饲粮循环(预试期9 d,收集期6 d)。试验结果:(1)3个茶渣样的粗蛋白质(CP)、粗纤维(CF)、单宁(单宁酸等价,TAE)与CT含量范围分别为23.23%~28.11%、16.68%~17.84%、1.98%~2.10%和1.47%~1.57%。(2)茶渣/大豆粕混合料的rdDM、tdDM、rdCP、tdCP均随茶渣比例提高而线性下降,变化范围相应为61.39%~25.67%、79.61%~26.47%、60.76%~16.68%和84.85%~17.69%。(3)体内试验中,高比例茶渣影响采食量,饲粮D(P<0.01)、C(P>0.05)的DM与有机物质(OM)采食量低于A、B;RTLCT对DM、OM、中性洗涤纤维、酸性洗涤纤维、氮、钙和磷的消化率无显著影响(P>0.05);但随RTLCT水平提高尿氮排出量线性下降,导致氮存留率上升,C的氮存留率较A提高23.61%(P<0.05),B、D分别提高12.57%和15.88%(P>0.05);随RTLCT提高,瘤胃液pH、总氮浓度无显著变化(P>0.05),而NH3N与血浆尿素氮(BUN)含量线性下降,C、D的NH3N分别是A的50.36%( P>0.05)和46.55%(P<0.05),BUN相应为A的68.04%与59.99%(P<0.05)。可见,茶渣是高蛋白质、低纤维物质,单宁含量为中等的物质;本试验中,RTLCT降低了饲粮的体外瘤胃CP降解率,但未影响体内全消化道消化率,且通过减少尿N排出而提高了氮存留率;但高比例RTLCT降低了绵羊采食量。显然,RTLCT 1.53 g·kg- 1DM 对饲粮蛋白质的保护效果较好,且对采食量无显著影响。

Abstract: The present experiment was conducted to determine the effects of tannin content in residue of tealeaves(RTL) on digestion and utilization of nutrients and metabolic parameters of nitrogen in sheep. The contents of nutrients and tannin in RTL were analysed; The in vitro degradability(rdDM、tdDM、rdCP、tdCP) of dry matter(DM) and crude protein(CP) of mixtures with six ratio of RTL to soybean meal(0:10, 1:9, 2:8, 3:7, 4:6, 10:0 ), which levels of RTL condensed tannin( RTLCT) of the mixtures were 0.00, 0.16, 0.31, 0.47, 0.63, 1.57 g·kg- 1DM, respectively, was measured by incubating in rumen fluid and rumen fluid + HClpepsin. To study the effects of RTLCT contents on digestion and utilization of nutrients and metabolic profiles of nitrogen in sheep, four High Mountain Finewool wethers (one and a half years old and average live weight of (29±2.55)kg) fitted with permanent rumen fistula and a 4×4 Latin square design with four cycles of diet each of 9 d for preliminary followed 6 d for sampling were used in this experiment. The contents of RTLCT in diets were 0.00(A), 0.77(B), 1.53(C), 2.30(D) g·kg -1DM . The results showed that: (1) the range of contents of CP, crude fiber(CF), TAE(tannic acid equivalent) and CT in three samples of TRL were 23.23%28.11%, 16.68%17.84%, 1.98%2.10%, 1.47%1.57%, respectively. (2) The degradabilities of DM and CP of the mixtures in rumen fluid and rumen fluid + HClpepsin(rdDM, tdDM, rdCP, tdCP) decreased linearly as the contents of RTLCT , the range of change were 61.39%25.67%, 79.61%26.47%, 60.76%16.68%, 84.85%17.69%, respectively. (3) In vivo, the intake of diet was affected by highraito of RTL, compared with diet A and B, the intakes of DM and organic matter (OM) of diet C (P>0.05) and diet D (P<0.01) were lower; the digestibilities of DM, OM, calcium (Ca), phosphorus (P), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of diets were unaffected by the contents of RTLCT(P>0.05). But the excretions of urinary nitrogen were decreased linearly and retention of N increased as the RTLCT level, the retention of nitrogen of diet B, C, D were raised by 12.57% (P>0.05), 23.61%(P<0.05) and 15.88%(P>0.05)than for diet A, respectively; there were no significant changes of the levels of pH and total nitrogen of rumen fluid (P>0.05) as the levels increasing of RTLCT, while the contents of NH3N and plasma urea nitrogen(BUN) linearly decreased, the levels of NH3N and BUN of diet C and D were 50.36 %(P>0.05) , 46.55%(P<0.05)and 68.04%, 59.99%(P<0.05)lower than that of diet A, correspondingly. It follows that residue of tealeaves were a matter with highprotein, lowfiber and middle content of tannin; under the condition of this experiment, the in vitro rdCP were decreased by RTL CT, but the digestibilities of CP in total gastrointestinal tract were unaffected by levels of RTLCT, and N retentions were increased owing to reducing of the excretion of N in urine as RTLCT; yet high ratio of residue of tealeaves in diet resulted in lowing of intake by sheep. Obviously, the protection effect of RTLCT with 1.53 g·kg- 1DM on protein was better, but no significant effect on the intake of diet.