鸡传染性法氏囊病病毒VP2基因突变对病毒体外复制的影响

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (11): 1577-1583.doi:

鸡传染性法氏囊病病毒VP2基因突变对病毒体外复制的影响

祁小乐1,2，高立2，吴关2，邓小芸2，3，余飞2，张礼洲2，秦立廷2，高玉龙2，
王永强2，高宏雷2，刘娣1，华育平1，王笑梅2

1. 东北林业大学博士后流动站黑龙江省农业科学院博士后工作站，哈尔滨 150086；2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室，哈尔滨150001；3. 黑龙江科技职业学院，双城 150111

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-11-25 发布日期:2011-11-25
通讯作者: 王笑梅

Influence of the Mutations of VP2 on the Replication Efficiency of Infectious Bursal Disease Virus in vitro

QI Xiaole1,2, GAO Li2, WU Guan2, DENG Xiaoyun2, 3, YU Fei2, ZHANG Lizhou2, QIN Liting2, GAO Yulong2, WANG Yongqiang2, GAO Honglei2, LIU Di1, HUA Yuping1, WANG Xiaomei2*

1. Postdoctoral Workstation of Heilongjiang Academy of Agricultural Science, Postdoctoral Station of Northeast Forestry University, Harbin 150086, China; 2. Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China; 3. Heilongjiang Vocational College of Science and Technology, Shuangcheng 150111, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25
Contact: WANG Xiaomei

摘要/Abstract

摘要： 为了进一步了解影响鸡传染性法氏囊病病毒(IBDV)复制效率和致病力的分子基础，作者在序列分析的基础上，利用反向遗传操作技术，针对VP2上2个重要的氨基酸位点（222和279），修饰和拯救了相应的2株突变病毒，并研究了突变病毒在体内外的生物学特性。复制动力学数据显示，VP2的222位由酪氨酸突变为脯氨酸可将IBDV在鸡胚成纤维细胞（CEF）上的复制滴度提高6倍以上，而279位氨基酸对病毒复制效率无显著影响。SPF鸡的感染试验显示，222位和279位氨基酸与IBDV的致病力没有关系。本研究首次报道了VP2 222位和279位氨基酸与IBDV复制效率和致病力的关系，有助于更加全面地了解IBDV的基因功能，也将为新型IBDV疫苗的设计提供依据和思路。

Abstract: To study the molecular basis for the replication efficiency and virulence of infectious bursal disease virus (IBDV), two amino acid mutations (A222P, D279N) was introduced in to VP2 of IBDV, respectively, based on the information of sequence analysis. Using the reverse genetic research system, two responding mutated strains of IBDV were rescued and their biological characteristics were studied. The replication kinetic curves showed that the rescued virus including A222P replicated over 6 times higher than its parent strain did, while D279N couldn’t. Animal experiment of SPF chickens showed that residue 222 and 279 of VP2 did not contribute to virulence of IBDV. The relations between the two residues of VP2 (222, 279) and the replication and virulence of IBDV were reported firstly. The findings provide necessary information for further understanding the gene function of IBDV and the design of new tailored IBDV vaccines.

祁小乐;高立;吴关;邓小芸;余飞;张礼洲;秦立廷;高玉龙;王永强;高宏雷;刘娣;华育平;王笑梅. 鸡传染性法氏囊病病毒VP2基因突变对病毒体外复制的影响[J]. 畜牧兽医学报, 2011, 42(11): 1577-1583.

QI Xiaole;GAO Li;WU Guan;DENG Xiaoyun;;YU Fei;ZHANG Lizhou;QIN Liting;GAO Yulong;WANG Yongqiang;GAO Honglei;LIU Di;HUA Yuping;WANG Xiaomei . Influence of the Mutations of VP2 on the Replication Efficiency of Infectious Bursal Disease Virus in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(11): 1577-1583.

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