嗜群血蜱和长角血蜱ITS-2、COⅠ和COⅡ基因序列变异与亲缘关系分析

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (6): 746-754.doi:

嗜群血蜱和长角血蜱ITS-2、COⅠ和COⅡ基因序列变异与亲缘关系分析

古小彬^1#，余增莹^1#，杨光友¹*，孙家刚²，魏洪²，李开均²，王淑贤¹

1.四川农业大学动物医学院，雅安 625014；2.四川苍溪县畜牧局，苍溪 628400

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-06-25 发布日期:2010-06-25
通讯作者: 杨光友

Genetic Relationship and Sequence Variation of Haemaphysalis longicornis and H. conicinna based on ITS-2, COⅠ and COⅡ Gene Sequences

GU Xiao-bin^1#, YU Zeng-ying^1#, YANG Guang-you¹*, SUN Jia-gang²,
WEI-Hong², LI Kai-jun², WANG Shu-xian¹

1. College of Veterinary Medicine, Sichuan Agricultural University, Ya′an 625014, China;2. Cangxi Animal Husbandry Bureau, Cangxi 628400, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-06-25 Published:2010-06-25

摘要/Abstract

摘要： 本研究旨在阐明长角血蜱与嗜群血蜱间的亲缘关系。采用聚合酶链式反应（PCR）技术从长角血蜱和嗜群血蜱基因组DNA中扩增得到核糖体第二内部转录间隔区基因(ITS-2)片段、线粒体细胞色素氧化酶亚基Ⅰ基因 (COⅠ)片段和线粒体细胞色素氧化酶亚基II基因(COⅡ)片段，并进行测序，进而分析其基因变异与系统发育。结果表明，长角血蜱和嗜群血蜱的ITS2、COⅠ和COⅡ基因片段的大小存在差异，长角血蜱3种基因分别为361、479和647 bp，嗜群血蜱基因分别为354、474和661 bp。长角血蜱与嗜群血蜱间ITS-2、COⅠ和COⅡ基因的相似性分别为84.2%、89.0%和88.4%。以3种基因构建的NJ进化树中，长角血蜱和嗜群血蜱均聚类。因此，认为长角血蜱和嗜群血蜱间ITS-2、COI和COII基因间变异较小，它们是血蜱属中亲缘关系较近的2个有效种，支持传统形态学的分类地位。

Abstract: This experiment was conducted to clarify the genetic relationship between Haemaphysalis longicornis and H. conicinna. The second internal transcribed spacer (ITS-2) of the rRNA gene, cytochrome c oxidase subunit Ⅰ (COⅠ) and cytochrome c oxidase subunit Ⅱ (COⅡ) of the mitochondrial gene were sequenced. Then, the nucleotide variability of the genes between the two tick species was compared and phylogenetic relationship was analyzed. The lengths of ITS-2, COⅠ and COⅡ in H. longicornis were 361, 479 and 647 bp, and those in H. conicinna were 354, 474 and 661 bp, respectively. The gene homology of ITS-2, COⅠ and COⅡ between H. longicornis and H. conicinna were 84.2%, 89.0% and 88.4%, respectively. Phylogenetic trees reconstructed using the NJ method based on genes ITS-2, COⅠ and COⅡ indicate that H. longicornis and H. conicinna are clustered in the same clade. The results suggest that the length of ITS-2, COⅠ and COⅡ of H. longicornis and H. conicinna are different, and H. longicornis and H. conicinna should be two different valid species with close relationship in genetics, which supports the morphologic taxonomic status of H. longicornis and H. conicinna.

古小彬#;余增莹#;杨光友;孙家刚;魏洪;李开均;王淑贤. 嗜群血蜱和长角血蜱ITS-2、COⅠ和COⅡ基因序列变异与亲缘关系分析[J]. 畜牧兽医学报, 2010, 41(6): 746-754.

GU Xiao-bin#;YU Zeng-ying#;YANG Guang-you;SUN Jia-gang;WEI-Hong;LI Kai-jun;WANG Shu-xian . Genetic Relationship and Sequence Variation of Haemaphysalis longicornis and H. conicinna based on ITS-2, COⅠ and COⅡ Gene Sequences[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(6): 746-754.

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