Abstract: The partial fragments of GH gene polymorphisms of 90 Nanjiang Brown goats were detected by PCR-RFLP.Association of PCR- RFLP at the GH gene with early growth and developmental traits in the Nanjiang Brown goats was also analyzed. The result showed that the sequence of PCRamplified products was 985 bp which included goat GH gene sequences of exon 3, exon 4, exon 5 and intron 3, intron 4. The PCR products were digested by two kinds of restriction endonuclease named FokⅠ and AciⅠ, respectively. Both of them had polymorphisms.The FokⅠ produced GG, GA and AA genotypes which frequencies were 0.522, 0.289 and 0.189, but the allele frequency of G (0.666) was higher than that of A (0.334).The AciⅠ produced CC, TC and TT genotypes which frequencies were 0.467，0.322 and 0.211， respectively, but the allele frequency of C (0.628) was higher than that of T (0.372).Chi-square test revealed that the genotype frequencies produced by restriction endonucleases were non-equilibrium Hardy-Weinberg. Association analysis of PCR-RFLP at the GH gene with early growth and developmental traits indicated that the least square means of weight, body height and body length with GA and TC genotypes were both significantly higher than that with other genotypes at different month ages (0.05<P<0.01 or P<0.01)