Abstract: P1 gene of swine vesicular disease virus (SVDV) was amplified by PCR using specific primers from SVDV HK/70 genome.The amplified fragment was cloned into pBABE puro vector for sequence analysis,and the recombinant plasmid was named pBABE puro-P1. Then pBABE puro-P1 and pVSV-G were cotransfected into GP2-293 packaging cells by liposomes, and recombinant retrovirus was acquired.The recombinant retroviruses was transfected into PK-15 cell by polybrene. The transfectants were selected by paromycin.Results of immunofluorescence, PCR analysis and Western blot showed that the foreign gene was integrated into the chromosome of transfected PK-15 cells and the expressed protein could react with positive serum against SVDV.