Abstract: To investigate their anti-inflammatory mechanism, the effects of berberine (Ber.), chlorogenic acid (Ch. A.) and baicalin (Bai) on the expression of intercellular adhesion molecule 1 (ICAM-1) in rat intestinal mucosa microvascular endothelial cells (RIMMVECs) challeged by lipopolysaccharide (LPS) were analyzed by immunocytochemical staining, and gray scale and statistical analysis were performed. The results showed that RIMMVECs constitutively express ICAM-1 in low level and LPS significantly increased it, while LPS-induced strong expression of ICAM-1 in RIMMVECs was suppressed by Ber, Ch- A and Bai. The results indicated that the anti-inflammatory role of Ber, Ch- A and Bai is related to their downregulating effect on LPS-mediated ICAM-1 expression, which provide data for clarifying their mechanism.