Abstract: This study conducted in-depth research on the effect factors of the growth of reconstructed embryos, through comparing the different serum starvation days, the number of generations with the recovery of cryopreserved of Donor fibroblast and so text conditions. The research results show that the difference of reorganized embryo cleavage rate is not significant (P> 0.05) as donor serum were starved 0, 1-3, 4-6 and 7-9 days, but blastocysts rate was the highest as donor serum were starved 1-3 days, and the difference was significant (P＜0.05) compared with the 4-6 and 7-9 days groups; the difference of embryo cleavage rate wasnot significant(P>0.05) and morula rate of 1-3 generation was the highest as the cells of 0、1-3、4-6 and 7-9 generation as a source of donor cells; The cleavage rate of reorganized embryos from 2 generation frozenthawed cell was significantly higher compared with 4 and 8 generation. 2 and 6 generation thawed cells of after frozen as were donor cells, egg(embryo) cleavage rate did not differ significantly, and morula rate of 8 generation was lower compared with the other groups. This experiment provide important theoretical basis and technological support to improve donor cells re-program(procedure) in oocyte, the growth of follow-up reorganization embryo and the research of related fields.