Abstract: According to the published sequences, three siRNAs (Small interference RNA) were designed, and expression vectors were constructed and transfected into goose granulosa cells cultured in vitro. The expression level of Bcl-2 protein, apoptosis and proliferation of granulosa cells were analyzed by flow cytometry 48 h after the transfection, and the progesterone (P) in the medium was assayed using RIA. Meanwhile, apoptosis indexes and Bcl-2 protein level of follicular granulosa cells with different rank were also analyzed. Our results indicated that: (1) Levels of Bcl-2 protein in follicular granulosa cells with different rank were different, the highest expression level was observed in the smallest preovulatory follicle (SPY), and levels of Bcl-2 protein in the healthy follicles were significant higher than that in atresic follicles (P < 0.05); (2) Bcl-2 protein levels of test groups were significant lower than that of control (P < 0.05), while apoptosis indexes (AI), proliferation indexes (PI) and P secretion of test groups were higher than that of control (P < 0.05).