Abstract: The 5′UTR of CAPN3 (Calpain3) gene was detected for searching SNPs and analyzing the distribution rule of different genotypes in different breeds, in order to study the relationship between CAPN3 gene and meat quality, meanwhile, searching the markers correlated with meat quality. SNPs were screened by sequencing and different genotypes were determined by polymerase chain reaction restriction fragment length polymorphism (PCRRFLP), then allele frequencies and polymorphism information content (PIC) were calculated. The result indicated that a new SNP (C→T) was found at 2 546 bp. The analysis result of the relationship between genotypes and productive performances by the Least Square Method (LSM) showed that the difference between AB and BB genotypes in carcass weight was significant (P<0.05). CAPN3 gene was primarily deduced to be a potential major gene or linked to major gene effecting meat quality traits. And this SNP might be a potential molecular marker of MAS.