中药有效部位组方对高温刺激小鼠肠道淋巴细胞体外增殖的影响

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (3): 360-364.doi:

中药有效部位组方对高温刺激小鼠肠道淋巴细胞体外增殖的影响

王自力;董虹;刘凤华;毛帅;刘仁志;许剑琴

1中国农业大学动物医学院，北京 100094；2西南大学动物科技学院，重庆 400715；3北京农学院动物科学系，北京 102206

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-03-24 发布日期:2008-03-24

Effect of Chinese Medicine Effective Component Prescription on in vitro Proliferation of Mouse Intestinal Lymphocyte after Stimulation with High Temperature

WANG Zi-li;DONG Hong;LIU Feng-hua;MAO Shuai;LIU Ren-zhi;XU Jian-qin

1College of Veterinary Medicine, China Agricultural University, Beijing 100094, China;2 College of Animal Science and Technology, Southwest University, Chongqing 400715, China;3 Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-03-24 Published:2008-03-24

摘要/Abstract

摘要： 根据中兽医“君、臣、佐、使”的组方原则和正交试验设计法将藿香、苍术、黄柏和石膏4味中药的有效部位按照不同剂量组合成8个不同中药有效部位复方，利用MTT法检测高温刺激小鼠肠道淋巴细胞增殖情况，探讨各个复方对高温刺激小鼠肠道淋巴细胞体外增殖的影响及其组方机制。结果表明：以中药有效部位复方A1B1C1D1（黄柏生物碱、藿香挥发油、苍术挥发油各200 μg/mL，石膏水提物100 μg/mL）对高温刺激小鼠肠道淋巴细胞体外增殖效果最优，复方中以藿香挥发油和黄柏生物碱为主药，苍术挥发油和石膏水提物为臣、佐药，以藿香挥发油和苍术挥发油交互作用较好。

Abstract: The effective components of herba agastachis, rhizoma atractylodis, gypsum fibrosum and cortex phellodendri were composed in different combination according to the principal of traditional Chinese composing prescription and orthogonal design to form 8 prescriptions. The effects of different prescriptions on in vitro proliferation of mouse intestinal lymphocyte after stimulation with high temperature were studied. The results showed that herba agastachis aetherolea and cortex phellodendri alkaloid were the major drugs in the prescription, rhizoma atractylodis aetherolea and gypsum fibrosum water extract were adjuvant drug and messenger drug. The prescription composed of 200 μg/mL herba agastachis aetherolea, cortex phellodendri alkaloid and rhizoma atractylodis aetherolea, 100 μg/mL gypsum fibrosum water extract, has the best effect on the proliferation of intestinal lymphocyte, and the interaction of herba agastachis and rhizoma atractylodis aetherolea was better.

王自力;董虹;刘凤华;毛帅;刘仁志;许剑琴. 中药有效部位组方对高温刺激小鼠肠道淋巴细胞体外增殖的影响[J]. 畜牧兽医学报, 2008, 39(3): 360-364.

WANG Zi-li;DONG Hong;LIU Feng-hua;MAO Shuai;LIU Ren-zhi;XU Jian-qin. Effect of Chinese Medicine Effective Component Prescription on in vitro Proliferation of Mouse Intestinal Lymphocyte after Stimulation with High Temperature[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(3): 360-364.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2008/V39/I3/360