Abstract: The experiment was conducted with the objective of examining the effect of dietary high copper on the cell cycle and apoptosis of kidney in ducklings by the methods of flow cytometry (FCM). 360 one-day-old Tianfu meat ducklings were randomly divided into six groups and fed on diets as follows: Control (Cu 8 mg/kg) and high copper (Cu 100 mg/kg，high copper groupⅠ；Cu 200 mg/kg，high copper groupⅡ；Cu 400 mg/kg，high copper group Ⅲ；Cu 600 mg/kg，high copper group Ⅳ；Cu 800 mg/kg，high copper groupⅤ) for six weeks. Kidney samples were collected, then the cell cycle and apoptosis were tested by FCM. The results showed that the percentage of renal cell apoptosis was increased in high copper groups Ⅲ, Ⅳ and Ⅴ when compared with that of control group. The G0/G1 phase of cell cycle of renal cell was lower, and S, G2+M phase was higher in high copper groupsⅠand Ⅱ than that in control group. The G0/G1 phase of cell cycle of renal cell was much higher, and S, G2+M phase was lower in high copper groups Ⅲ, Ⅳ and Ⅴthan that in control group ( P<0.05 or P<0.01). It is concluded that dietary copper in excess of 400 mg/kg diet impaired the progression of renal cells from G0/G1 phase to S phase obviously and induced renal cell apoptosis.