Abstract: Three overlapping cDNA fragments covering the approximate FMDV full genome (7.5kb）of the 3′ end viral genome were amplified using long RTPCR and were cloned into pBluescriptSK+ vector with unique restriction sites, respectively. A fragment (about 0.7 kb ) including 15 C of the 5′ end viral genome was amplified by over-lap PCR and was cloned into pGEM-T vector. All positive clones were assembled into a low copy number vector pCDNA31/Zeo（+）which was removed T7 promoter in the vector sequence and constructed FMDV Asia1/JS/China/2005 full-length cDNA clone. RNA was synthesized in vitro using T7 polymerase, the infective virus was obtained by transfecting the RNA into BHK-21 cells. The rescued virus was identified by the RT-PCR, indirect immunofluorescence, electron microscope and sulk mice pathogenicity, the results showed infectious FMDV was rescued successfully. The full-length infectious cDNA clone will lays the basis for elucidating the mechanism of pathogenesis of FMDV and developing novel vaccines against FMD.