Abstract: This experiment was conducted to determine immunity protection effectiveness of fimC protein from Avian Pathogenic Escherichia coli(APEC) after fimC protein was expressed. fimC gene was amplified from APEC O2, and then was cloned into pMD18-T vector and sequenced. The results showed that fimC gene was 657 bp and encoded 218 amino acids. The sequence of fimC gene was analyzed by homology comparing with that of human, they shared 97.9% homology in nucleotide and 96% in amino acid. The fimC gene was sub-cloned from pMD18-T vector into the plasmid vector pET-30c to construct a recombinant expression plasmid pET-30c-fimC, which was transformed into E. coli BL21. Then a fusion protein about 25 kDa was expressed by inducing with IPTG, and the expressed protien was proved to be antigenic by Western Blot analysis. The laying hens were immunized with the expressed fimC protein to prepare high yolk antibodies of fimC. One-day-old healthy chickens were immunized with the yolk antibodies of fimC, and injected with APEC next day, results showed that the mortality of immunized group and the control group was 60% and 70% respectively. The results indicated that fimC yolk antibodies could provide protection to some extent against the attacks of APEC.