Abstract: According to the published nucleotide sequence of giardiavirus, six pairs of primers were designed and total nucleic acids from trophozoites of Giardia canis isolated from dogs in Changchun were used as template for RT.PCR. After RT.PCR, the products were linked into pMD18T vector, then cloned ,sequenced and analyzed. The genome sequence of Giardia canis virus was 6 276bp(DQ238861)，which revealed the presence of two large open reading frames that are separated by a -1 frameshift and share an overlap of 220 nt. The ORF1(367-3030) encodes a polypeptide of 887 amino acid residues. The ORF2(2808-5981) encodes a polypeptide of 1 056 amino acid residues,which contains all consensus RNA-dependent RNA polymerase sequence motifs. The ratio of G+C in the genome was 49.62%. The homology of Giardia canis virus to sequence of L13218(GenBank) was 94.62% in the nucleotide, and 93.50% in the amino acid levels. Compared with the sequences of AF525216(GenBank), the homology was 98.88% in the nucleotide, and 98.30% in the amino acid levels.