Abstract: To construct the prokaryotic and eukaryotic expression vector and prepare the antiserum of peroxisome proliferator-activated receptor-gamma (PPAR-γ) gene in chicken. The sequence of PPAR-γ gene was amplified by reverse transcriptase-PCR(RT-PCR) from the total RNA of chicken fatty acid and insert into pGEX-4T-1 vector and pcDNA3 vector. Recombinant plasmid pGEX-4T-1/PPAR-γ was expressed in E.coli BL21; Mice were immunized with purified recombinant plasmid pcDNA3/PPAR-γ. SDS-PAGE showed that recombinant pGEX-4T-1/PPAR-γ expressed abundantly a fusion protein in E.coli BL21. ELISA and Western blot showed that antibody obtained from the serum of mice by injecting recombinant pcDNA3/PPAR-γ was high effective and strong specific. The recombinant pcDNA3/PPAR-γ in the current study would provide a powerful tool for overexpression research of chicken PPAR-γ gene at cell level; The recombinant protein and antiserum in the current study would also establish a basis for function research of chicken PPAR-γ at protein level.