Abstract: To construct an inhinbin (INH) expression vector with high immunogenicity, INHα1-32 gene was incorporated into the S gene of pcIS between amino acid residues 112 and 113. The resultant plasmid pcISI with two copies of INHα_1-32 was identified by restriction endonuclease digestion and sequencing. The plasmid pcISI was then introduced into HeLa cells via liposome-mediated transfection and the immunogenicity of recombinant protein was detected by ELISA. The results showed that the recombinant gene expressed in HeLa cells, and the INH immunogenicity of ISI fusion protein was higher than that of IS fusion protein, indicating the ISI expression vector was constructed successfully. Five of six rats immunized with pcISI produced antibodies against INH in vivo. In addition, the P/N values of antibodies were higher than those immunized with pcIS from 2nd week to 6th week. In conclusion, the constructed pcISI could express INH with high immunogenicity.