临床分离的动物源链球菌对红霉素 的蓄积动力学研究

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (2): 202-205.doi:

临床分离的动物源链球菌对红霉素的蓄积动力学研究

王丽平;陆承平

南京农业大学动物医学院，南京 210095

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25
通讯作者: 王丽平

Accumulation dynamics of erythromycin in Streptococcus strains isolated from veterinary clinic

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要/Abstract

摘要： 为证实国内临床分离的动物源链球菌菌株中是否存在与红霉素耐药性相关的主动外排机制，选取含有mefA基因、ermB基因、不含mefA /ermB基因的链球菌耐药菌株和青霉素、红霉素均敏感菌株，采用完整细胞物质转运动力学方法研究了菌株对红霉素的主动外排功能。结果表明，含mefA基因的菌株对红霉素的主动外排作用显著增强，对红霉素的蓄积浓度显著低于其它菌株（P<0.05），且CCCP可减少红霉素的主动外排作用，增加菌体内红霉素的蓄积浓度(P<0.05)。本试验结果佐证了在链球菌内存在与红霉素耐药相关的主动外排机制。

Abstract: To identify if there was the mechanism of active efflux in erythromycin resistant Streptococcus strains with mefA gene isolated from veterinary clinic，several erythromycin resistant strains with mefA gene, ermB gene and several susceptible strains were selected in this test. The uptake of 14C-erythromycin in all strains and the effects of carbonylcyanidem chlorophenylhydrazone (CCCP, a proton conductor,) on the erythromycin accumulation were assayed with intact cells. The results showed that increased active exocytosis and decreased accumulation of erythromycin (P<0.05) were occurred in strains with mefA gene, and CCCP led reverse results. The accumulation and the response to the addition of a proton conductor indicated that the erythromycin resistant strains with mefA gene could actively pump out 14C-erythromycin from the cell.

王丽平;陆承平. 临床分离的动物源链球菌对红霉素的蓄积动力学研究[J]. 畜牧兽医学报, 2007, 38(2): 202-205.

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