高钼对艾维茵肉鸡法氏囊细胞周期和凋亡影响分析

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (8): 1031-1038.doi:

高钼对艾维茵肉鸡法氏囊细胞周期和凋亡影响分析

杨帆，崔恒敏*，肖杰，彭西，崔伟，程安春，陈涛，柏才敏

四川农业大学动物医学院环境公害与动物疾病四川省高校重点实验室，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-08-20 发布日期:2010-08-20
通讯作者: 崔恒敏

Effect of Dietary High Molybdenum on the Cell Cycle andApoptosis of Bursa of Fabricius in Broilers

Key Laboratory of Environmental Hazard and Animal Diseases of Sichuan Province,College of Veterinary Medicine, Sichuan Agricultural University, Ya′an 625014, China

Key Laboratory of Environmental Hazard and Animal Diseases of Sichuan Province,College of Veterinary Medicine, Sichuan Agricultural University, Ya′an 625014, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20

摘要/Abstract

摘要： 300只1日龄艾维茵肉鸡健雏随机分为4组，分别饲以对照日粮（Mo 13 mg·kg^-1）和高钼日粮(Mo 500 mg·kg^-1，高钼Ⅰ组；Mo 1 000 mg·kg^-1，高钼Ⅱ组；Mo 1 500 mg·kg^-1，高钼Ⅲ组)6周，以实验病理学和流式细胞术的方法观察雏鸡法氏囊变化。结果，高钼Ⅱ、Ⅲ组法氏囊绝对质量和脏器指数显著低于对照组。组织学观察见法氏囊淋巴细胞减少，网状细胞增生。超微结构观察，淋巴细胞线粒体肿胀、嵴断裂，或见线粒体基质电子密度增高；凋亡淋巴细胞增多。流式细胞结果显示，28和42日龄时高钼Ⅱ、Ⅲ组法氏囊淋巴细胞G0/G1期显著升高（P<0.01），S期、G2+M期和增殖指数（PI）显著降低（P<0.01），同时凋亡率显著高于对照组（P<0.01），TUNUL染色观察结果与流式细胞仪检测结果一致。结果表明，日粮钼含量为1 000及1 500 mg·kg^-1时可抑制法氏囊生长发育。

Abstract: The experiment was conducted with the objective of examining the effects of high molybdnum on the bursa of Fabricius in broilers by the methods of experimental pathology and flow cytometry (FCM).300 one-day-old Avian broilers were divided into four groups and fed on Control diet (Mo 13 mg·kg^-1) and High molybdenum diets(Mo 500 mg·kg^-1, High molybdenum groupⅠ; Mo 1 000 mg·kg^-1, High molybdenum groupⅡ; Mo 1 500 mg·kg^-1, High molybdenum group Ⅲ) for 6 weeks. The result showed that the weight and the relative weight of bursa of Fabricius were decreased, lymphocytes were histopathologically decreased and reticulocytes were increased in number in high molybdenum groupⅡand Ⅲ. Ultrastructurally, the frequency of lymphocyte apoptosis was higher in high molybdenum group Ⅱ and Ⅲ than that in control group. The mitochondria were swelled and mitochondria cristas were broken, or the density of mitochondrial matrix was increased. The statistical analyses by FCM indicated that the G0/G1 phase was increased (P<0.01) and the G2+M phase, S phase and the PI (Proliferating index) were decreased (P<0.01) of bursa in high molybdenum group Ⅱ and Ⅲ at 28 and 42 days of age. Meanwhile, the percentage of cellular apoptosis was higher in high molybdenum group Ⅱ and Ⅲ than that in control group. Also, the TUNEL staining was consistent with the result of FCM. It was concluded that dietary molybdenum in 1 000 and 1 500 mg·kg-1 inhibited the development of the bursa of Fabricius, caused pathological changes in the bursa of Fabricius in broilers.

杨帆;崔恒敏;肖杰;彭西;崔伟;程安春;陈涛;柏才敏. 高钼对艾维茵肉鸡法氏囊细胞周期和凋亡影响分析[J]. 畜牧兽医学报, 2010, 41(8): 1031-1038.

Key Laboratory of Environmental Hazard and Animal Diseases of Sichuan Province;College of Veterinary Medicine;Sichuan Agricultural University;Ya′an 0;China. Effect of Dietary High Molybdenum on the Cell Cycle andApoptosis of Bursa of Fabricius in Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(8): 1031-1038.

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