畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (9): 969-973.doi:

• 研究简报 • 上一篇    下一篇

胸膜肺炎放线杆菌的实时荧光PCR检测

李树清;易建平;李 健;胡永强;王巧全;陈志飞;周筱华;潘晓钟;罗满林; 陈 敏   

  1. 1. 上海出入境检验检疫局，上海 200135; 2.华南农业大学兽医学院，广州 510642
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-09-25 发布日期:2005-09-25

The Detection of Actinobacillus pleuropneumoniae by Real-time PCR

LI Shu-qing;YI Jian-ping;LI Jian;HU Yong-qiang;WANG Qiao-quan;CHEN Zhi-fei;ZHOU Xiao-hua;PAN Xiao-zhong;LUO Man-lin;CHEN Min
  

  1. 1. Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135,China；2. South China Agricultural University, Guangzhou 510642,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-09-25 Published:2005-09-25

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Abstract: Primers FP/ RP and TaqMan-MGB Probe were designed from sequence of apxIVA gene specific to all serotypes of Actinobacillus pleuropneumoniae. A real-time PCR method was developed for detecting Actinobacillus pleuropneumoniae, which can amplify all strains of APP. The sensitivity is 12fg DNA or 5.1 CFU bacteria. This method can be directly used for detection of APP without DNA extraction.

ISSN 0366-6964
CN 11-1985/S
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