蚯蚓寡肽对伪狂犬病毒感染细胞和HeLa细胞凋亡的影响

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (9): 942-946.doi:

蚯蚓寡肽对伪狂犬病毒感染细胞和HeLa细胞凋亡的影响

刘艳琴;孙振钧;张国中;李世杰

1.中国农业大学资源与环境学院，北京 100094； 2.河北农业大学动物科技学院，保定 071001；3. 中国农业大学实验动物研究所，北京 100094

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-09-25 发布日期:2005-09-25

Effects of Earthworm Oligo-peptides on the Infection to Cell of PRV and HeLa Cell Apoptosis

LIU Yan-qin; SUN Zhen-jun;ZHANG Guo-zhong; LI Shi-jie

1. College of Resources and Environmental Science, China AgriculturalUniversity, Beijing 100094, China；2. College of Animal Science, HebeiAgricultural Universtiy, Baoding 071000, China;3. Institute of Laboratory AnimalScience, China Agricultural University, Beijing 100094, China

Received:1900-01-01 Revised:1900-01-01 Online:2005-09-25 Published:2005-09-25

摘要/Abstract

摘要： 利用细胞病变抑制法测定了蚯蚓寡肽的体外抗伪狂犬病毒（PRV）作用，分别用AO/EB染色法和DNA琼脂糖凝胶电泳法测定了蚯蚓寡肽对HeLa细胞凋亡的影响。结果显示，蚯蚓寡肽可以抑制PRV感染细胞，同时存在剂量效应和时间效应。蚯蚓寡肽浓度越大细胞病变程度越轻，各处理组在72 h的细胞病变程度最轻。蚯蚓寡肽可以诱导HeLa细胞凋亡, 且具有浓度效应，1.5 mg/mL组、0.75 mg/mL组的细胞凋亡率分别为60.20%和48.12%。

Abstract: In this paper, the CPE inhibition test was used to detect the antivirus effects, and the apoptosis effects on HeLa cell was studied by AO/EB staining method and DNA agarose electrophoresis. The results showed that the oligo-peptide could inhibit PRV infecting cells. At the same time, the effect had a dose and time dependent effect. The higher the concentration was, the smaller the CPE degree was, and the lowest CPE occurred when treated for 72 h. It verified that oligopeptide could lead to apoptosis of HeLa cell. The apoptotic effect was dose dependent. The apoptotic rates of HeLa cell in the 1.5mg/mL, 0.75 mg/mL group were 60.20% and 48.12% respectively.

刘艳琴;孙振钧;张国中;李世杰. 蚯蚓寡肽对伪狂犬病毒感染细胞和HeLa细胞凋亡的影响[J]. 畜牧兽医学报, 2005, 36(9): 942-946.

LIU Yan-qin;SUN Zhen-jun;ZHANG Guo-zhong;LI Shi-jie. Effects of Earthworm Oligo-peptides on the Infection to Cell of PRV and HeLa Cell Apoptosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(9): 942-946.

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