亚洲Ⅰ型口蹄疫病毒双拷贝VP1基因的串联表达及其免疫原性

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (3): 383-387.doi:

亚洲Ⅰ型口蹄疫病毒双拷贝VP1基因的串联表达及其免疫原性

张克山^1，2，向敏^1，2，吴斌^1，2*，王勤刚^1，2，陈焕春^1，2

1.华中农业大学动物医学院动物病毒实验室，武汉 430070；2.华中农业大学农业微生物学国家重点实验室，武汉 430070

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-24 发布日期:2009-03-24
通讯作者: 吴斌

Tandem Expression and Immunogenicity of Two Copies VP1 Gene of FMDV Serotype Asia 1

ZHANG Ke-shan^1,2， XIANG Min^1,2，WU Bin^1,2*, WANG Qin-gang^1,2，CHEN Huan-chun^1,2

1.Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China；2.National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-24 Published:2009-03-24

摘要/Abstract

摘要： 口蹄疫病毒（FMDV）VP1基因含有T细胞和B细胞表位，是口蹄疫病毒的主要免疫原性基因。作者将亚洲Ⅰ型FMDV VP1基因首尾串联构建双拷贝的VP1基因（2VP1），实现双拷贝VP1基因的原核表达，表达的重组蛋白（GST2VP1）经Sephadex-G200分子筛层析纯化后，Western blot证实其有反应原性，动物试验表明重组蛋白在加强免疫后能够产生和灭活疫苗相当的ELISA抗体和中和抗体；本试验结果为亚洲Ⅰ型FMDV免疫原性研究及GST2VP1蛋白的进一步应用奠定了基础。

Abstract: VP1 gene of foot-and-mouth disease virus (FMDV) is the major immunogenic gene as it contains T cell and B cell epitopes. In this study, we constructed a recombinant expressing plasmid(KG2VP1) including two VP1 genes(2VP1) of Asia 1 FMDV, and the 2VP1 gene was expressed in E.coli BL21. The fusional protein (GST2VP1) which was purified by Sephadex-G200 sieve chromatography demonstrated the reactionogenicity by Western blot. And the mice test indicated that the GST2VP1 can induce similar levels of ELISA and neutralization antibody like inactivated vaccine. We provide foundation for researching the immunogenicity of FMDV serotype Asia 1 and further application of GST2VP1 in future.

张克山;向敏;吴斌;王勤刚;陈焕春;. 亚洲Ⅰ型口蹄疫病毒双拷贝VP1基因的串联表达及其免疫原性[J]. 畜牧兽医学报, 2009, 40(3): 383-387.

ZHANG Ke-shan;XIANG Min;WU Bin;WANG Qin-gang;CHEN Huan-chun;. Tandem Expression and Immunogenicity of Two Copies VP1 Gene of FMDV Serotype Asia 1[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(3): 383-387.

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