奶牛PRL基因CRS-PCR多态性与产奶性状的关联分析

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (3): 303-308.doi:

奶牛PRL基因CRS-PCR多态性与产奶性状的关联分析

王丽娟^1,2，李秋玲¹，王长法¹，王洪梅¹，李建斌¹，侯明海¹，高运东¹，仲跻峰¹*

1.山东省农业科学院奶牛研究中心,济南 250100；2.山东大学公共卫生学院，济南 250012

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-24 发布日期:2009-03-24
通讯作者: 仲跻峰

CRS-PCR Polymorphisms of Prolactin Gene and Its Relationship with Milk Production Traits in Chinese Holstein Cows

WANG Li-juan^1,2，LI Qiu-ling¹，WANG Chang-fa¹，WANG Hong-mei¹，
LI Jian-bin¹，HOU Ming-hai¹，GAO Yun-dong¹，ZHONG Ji-feng¹*

1. Dairy Cattle Research Centre, Shandong Academy of Agricultural Sciences, Jinan 250100, China;2. School of Public Health,Shandong University,Jinan 250012, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-24 Published:2009-03-24

摘要/Abstract

摘要： 采用CRS-RFLP技术对中国荷斯坦牛PRL基因第4外显子C8377G位点及第4内含子T8510C位点进行单核苷酸多态性分析。最小二乘分析表明：在8377位点GG基因型个体产奶量最小二乘均值显著高于CC基因型（P<0.05），GC基因型个体乳蛋白率最小二乘均值显著高于CC基因型（P<0.05）；在8510位点CC基因型个体产奶量最小二乘均值显著高于TT基因型（P<0.05）。

Abstract: Single nucleotide polymorphisms of exon 4 C8377G and intron 4 T8510C of PRL gene were detected in Chinese Holstein cows by CRS-RFLP. At 8377 site,the least square mean of milk yield of this population was higher for individuals with genotype GG than that for individuals with genotype CC (P<0.05); The least square mean of protein percentage of this population was higher for individuals with genotype GC than that for individuals with genotype CC (P<0.05).At 8510 site, the least square mean of milk yield of this population was higher for individuals with genotype CC than that for individuals with genotype TT (P<0.05).

王丽娟;李秋玲;王长法;王洪梅;李建斌;侯明海;高运东;仲跻峰. 奶牛PRL基因CRS-PCR多态性与产奶性状的关联分析[J]. 畜牧兽医学报, 2009, 40(3): 303-308.

WANG Li-juan;LI Qiu-ling;WANG Chang-fa;WANG Hong-mei;LI Jian-bin;HOU Ming-hai;GAO Yun-dong;ZHONG Ji-feng. CRS-PCR Polymorphisms of Prolactin Gene and Its Relationship with Milk Production Traits in Chinese Holstein Cows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(3): 303-308.

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