GnRH-A免疫对兔去势效果的实验研究

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (2): 191-196.doi:

GnRH-A免疫对兔去势效果的实验研究

魏锁成¹*，巩转娣²

1.西北民族大学动物医学研究所，兰州 730030；2.西北民族大学医学院附属医院，兰州 730030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-24 发布日期:2009-02-24
通讯作者: 魏锁成

Experimental Research on Efficacies of GnRH-A Immunocastration in Rabbits

WEI Suo-cheng¹*，GONG Zhuan-di²

1.Animal Medicine Institute of Northwest University for Nationalities， Lanzhou 730030, China;2.Affilated Hospital of Medicine College Northwest University for Nationalities, Lanzhou 730030,China

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-24 Published:2009-02-24

摘要/Abstract

摘要： 探讨促性腺激素释放激素类似物（GnRH-A）对动物免疫去势的效果和作用机理。用自制的GnRH-A抗原乳剂在30只兔（EG-Ⅰ与EG-Ⅱ组）的颈背部皮下分2～3点注射1.0 mL（100 μg·mL^-1）GnRH-A抗原，EG-Ⅱ组3周后加强免疫1次。测定睾丸长度和质量，体质量变化，GnRH抗体效价与血清睾酮浓度。制备的抗原无菌性、安全性和物理性状良好，EG-Ⅱ组睾丸长度与EG-Ⅰ组差异极显著（P<0.01）；EG-Ⅱ组的抗体水平也明显高于EG-Ⅰ组；免疫注射后2个试验组与对照组的血清睾酮浓度差异逐渐增加，102 d 时EG-Ⅱ组血清睾酮浓度极显著低于对照组（P<0.01），且第28天后EG-Ⅱ组显著低于EG-Ⅰ组（P<0.05）；EG-Ⅱ组雄兔的体质量和平均日增质量均最大，显著高于EG-Ⅰ组和对照组（P<0.05）。GnRH-A免疫家兔对睾丸发育、血清GnRH抗体效价和睾酮浓度具有显著的影响，加强免疫效果更理想。

Abstract: To investigate the efficacies and mechanisms of immunocastration by using gonadotropin releasing hormone analogues (GnRH-A) in animals. The GnRH-A antigen emulsion was made by adding incomplete Freund′s adjuvant after GnRH-A was combined with bovine serum albumin(BSA). 30 rabbits were injected with GnRH-A antigen, the testis length and weight as well as body weight of the immunized rabbits were measured，GnRH antibody titer and testosterone concentration were detected through ELISA. There was a significant difference of the testis lengths between two EGs groups(P<0.01)，antibody levels of EG-Ⅱ group was higher obviously than that of EG-Ⅰ; the serum testosterone concentration between EG-Ⅰ and EG-Ⅱ groups were remarkably different from 7 to 16 weeks(P<0.05 )，the body weight and average daily gain of EG-Ⅱ group were the biggest and significantly higher than that of EG-Ⅰ group as well CG(P<0.05). The testis development，serum GnRH antibody titer and testosterone concentration of experiment rabbits changed obviously after immunization of GnRH-A and adding another injection would strengthen castration effects.

魏锁成;巩转娣. GnRH-A免疫对兔去势效果的实验研究[J]. 畜牧兽医学报, 2009, 40(2): 191-196.

WEI Suo-cheng;GONG Zhuan-di. Experimental Research on Efficacies of GnRH-A Immunocastration in Rabbits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(2): 191-196.

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