布鲁氏菌VirB8变异株的构建及其感染力和毒力的测定

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (6): 892-897.doi:

布鲁氏菌VirB8变异株的构建及其感染力和毒力的测定

钟旗^1,2，何倩倪¹，范伟兴³，谷文喜¹，
易新萍⁴，吴冬玲¹，叶锋¹，李博¹ ，刘丽娅¹

1.新疆畜牧科学院兽医研究所，乌鲁木齐 830000；2.东北农业大学动物医学学院，哈尔滨 150030；3.中国动物卫生与流行病学中心，青岛 266032；4.新疆自治区产品质量监督检验研究院，乌鲁木齐 830002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-06-26 发布日期:2009-06-26
通讯作者: 钟旗

Construction of VirB8 Deletion Mutants of Brucella and Detection of Its Infection and Virulence

ZHONG Qi ^1,2 , HE Qianni ¹, FAN Weixing³, GU Wenxi ¹,
YI Xinping ⁴,WU Dongling ¹，YE Feng ¹，LI Bo¹,LIU Liya ¹

1.Veterinary Research Institute,Xinjiang Academy of Animal Sciences,Urumqi 830000,China;2.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;3.China Animal Health and Epidemiology Center,Qingdao 266032,China;4.Xinjiang Product Quality Supervision and Inspection Academy,Urumqi 830002,China

Received:1900-01-01 Revised:1900-01-01 Online:2009-06-26 Published:2009-06-26
Contact: ZHONG Qi

摘要/Abstract

摘要： 作者拟对缺失致病力因子——四型分泌系统中的VirB8基因后布鲁氏菌感染力和毒力的变化进行测定与分析。首先，构建了布鲁氏菌VirB8基因缺失株（△B8B.suis），用缺失株感染巨噬细胞和BALB/c小鼠，再用B.suis强毒攻击BALB/c小鼠并检测脾脏含菌数，观察其在动物体内定居能力、毒力及抗感染保护力。鉴定△B8B.suis为VirB8基因完全缺失株，△B8B.suis感染巨噬细胞6、24和48 h，其CFU分别为49、165和355；△B8B.suis感染BALB/c小鼠的每克脾含菌数为36×107（1×108CFU腹腔接种）和5×106（1×109CFU蹊部接种）；BALB/c小鼠攻毒试验显示△B8Bsuis免疫组每克脾平均含菌数为761×102，非免疫对照组为298×105。结果表明布鲁氏菌缺失VirB8基因后，其毒力较亲本株弱，但能在小鼠脾脏定居，为弱毒株；△B8Bsuis呈现出作为疫苗的生物学特性，但毒株能否作为疫苗株使用，还需进一步验证。

Abstract: VirB8 deletion mutant of Brucella was constructed and named △B8B.suis. Then the virulence and potential protection efficacy of the △B8B.suis were evaluated by inoculating macrophages and BALB/c mouse. After culturing in macrophages, bacteria were recovered 6, 24, 48 h, and the mean CFU were 49, 165 and 355 respectively. Splenic CFU of BALB/c mouse were 36×107 (Mice was inoculated with 1×108CFU in abdominal cavity) and 56×105 (Mice was inoculated with 1×109CFU in inguinal region). After challenging, the splenic CFU of △B8B.suis immunization group was 761×102，while that of control group (No inoculation) was 298×105. The mutation of △B8B.suis was attenuated in macrophages and mouse model. It was able to colonize in spleen of BALB/c mouse and had some protective efficacy against Brucella suis. This approach provides a useful candidate vaccine for protecting animals from Brucella infection.

钟旗;何倩倪;范伟兴;谷文喜;易新萍;吴冬玲;叶锋;李博;刘丽娅. 布鲁氏菌VirB8变异株的构建及其感染力和毒力的测定[J]. 畜牧兽医学报, 2009, 40(6): 892-897.

ZHONG Qi;;HE Qianni;FAN Weixing;GU Wenxi;YI Xinping;WU Dongling;YE Feng;LI Bo;LIU Liya . Construction of VirB8 Deletion Mutants of Brucella and Detection of Its Infection and Virulence[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(6): 892-897.

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