Abstract:

In order to explore the possibility using SLADRA gene as genetic marker of disease resistance, the correlation between polymorphism of SLADRA gene exon 2 and piglets diarrhea were detected in 216 piglets from Landrace, Large White and Duroc pigs by PCRSSCP and sequencing. Three alleles and six genotypes were detected in the SLADRA gene exon 2. Six genotypes (AA, AB, BB, BC, AC and CC) were both found in Landrace and Large White pigs, but only four genotypes (AA, AB, BB and BC) were detected in the Duroc pigs. The genotype distribution was significantly different among Duroc and other two pig breeds (Landrace and Large White pigs) (P<0.01). The genotype distributions of three breeds were all in the HardyWeinberg equilibrium (P>0.05). The least square analysis indicated that genotype significantly affected the diarrhea of piglets (P<0.05), but the breed and gender showed no significant influence (P>0.05) on the diarrhea of piglets. The piglet diarrhea score of individuals with genotype AA and BB were significantly higher than that of individuals with genotype AC and CC (P<0.05). Therefore, it can be concluded that genotypes of SLADRA gene had significant influence on piglet diarrhea, and which might be a potential genetic marker in swine breeding.