Abstract: The objective of this study was to illuminate the molecular features and the expression profile of the porcine SRPK1 gene. The SRPK1 gene from the Yorkshire pig was cloned and sequenced, and then its molecular features were analyzed. Use inverse PCR to clone the partial 5′UTR sequence of SRPK1 in pig. The mRNA distribution profile of the porcine SRPK1 gene in ten tissues (heart, muscles, liver, kidney, lung, stomach, small and large intestine, spleen, brain) of Yorkshire and Duroc (one-day, one-month) were examined by real-time PCR. Construct pig skeletal muscle injury model by subcutaneous injection to study the expression characteristics of SRPK1 gene in repair process of skeletal muscle. A sequence with a length of 2 499 bp was cloned, it contained the CDS (coding sequence) region of 1 968 bp of the porcine SRPK1 gene, encoded a protein composed of 656 amino acids, including two P_Kc domains. The sequence of the porcine SRPK1 protein shared high similarity with human and chimpanzee, and they were closed in the Phylogenetic tree. The result of bioinformatics analysis showed that 5′UTR obtained in the study contained multiple transcription binding sites：HSF1, HSF2, Ik-1, IK-2, SRY, SP1 MyoD, USF, E47, p300, CP2, RREB-1, E2F and AP-1. The study results showed that, by realtime PCR, pigs had the organization- and species-specific in expression, but highly in stomach, small intestine and large intestine. The expression of SRPK1 gene fluctuated in the injury and repair process. There are several transcription binding sites in 5′UTR related to muscle development, it mainly expressed in digestive system and the expression level increased in injury and repair process of skeletal muscle. It was inferred that SRPK1 gene may be related to skeletal muscle cell development, or other growth-related factor expression.