Abstract: This experiment was conducted to study the effect of vitamin D on metabolism of calcium and phosphorus in Starter White Pekin Duckings. Seven hundred and twenty one-day-old male White Pekin Duckings with similar body weight were randomly allotted into 12 treatments with 6 repicates of 10 ducks each. Duckings were fed two levels of dietary calcium/phosphorus (0.8/0.4, 0.4/0.2) and six levels of vitamin D (0，250，500，1 000，2 000，3 000 IU·kg^-1) in a 2×6 factorial arrangement for 14 days. The results showed that: vitamin D in diets significantly effected the daily weight gain，daily feed intake and feed/gain (P<0.05). When the vitamin D was 2 000 IU·kg^-1, the plasma calcium reached the most level. With the increasing of vitamin D, plasma phosphorus and Alkline Phosphatase(AKP) activity were decreased signficantly (P<0.05). Tibia ash content increased when vitamin D increased from 500 to 1 000 IU·kg^-1 (P<0.05). More vitamin D was not effect any more (P>0.05). At the 2 000 IU·kg^-1 level of vitamin D, the calcium content in tibia ash reached its most level. Vitamin D in diets did not effect the phosphorus content in tibia ash. The results indicated that the vitamin D and calcium/phosphorus in diets significantly effected the growth performance, blood biochemistry and tibia parameter in White Pekin Duckings. The requirement of vitamin D was between 1 000 and 2 000 IU·kg^-1 for White Pekin Duckings of 14 days old.